Question

In a normal-phase liquid chromatographic column, a solute was found to have a retention time of 26.7 minutes, and an unretained component required 0.55 minutes for elution when the mobile phase was 40% (by volume) THF and 60 % acetonitrile.

Calculate: a) Retention factor (k)

b) the THF-acetonitrile composition that should bring k to a value of 4.

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Inorganic Chemistry

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