Use the data from the table below to answer the questions on an ezyme obtained from potato extract. The greater the absorbance value, the more product generated for each chemical reaction Table 1: Summary Data for Enzyme Catalyzed Reaction Product Formation Measured as Absorbance Tube Distilled Water Catechol PTU (Inhibitor) Potato Extract Results (Absorbance) 1 5.5 ml 0.5 ml -- --- 0.02 2 5 ml 0.5 ml --- 0.5 ml 0.68 3 4.5 ml 0.5 ml 0.5 ml 0.5 ml 0.04 4 4.5 ml 0.5 ml 1.0 ml 1.26 5 4.5 ml 1.0 ml 0.5 ml 1.22 a) Explain with respect to enzyme activity why test tube 1 produced very little absorbance: b) Explain with respect to enzyme activity why test tube 3 produced very little absorbance: c) Explain how to tubes 4 and 5 differ with respect to reaction conditions. d) Explain with respect to enzyme activity why tubes 4 and 5 have similar absorbance and why both have greater absorbance values than tube 2.
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
Use the data from the table below to answer the questions on an ezyme obtained from potato extract. The greater the absorbance value, the more product generated for each
|
Table 1: Summary Data for Enzyme Catalyzed Reaction Product Formation Measured as Absorbance |
|||||
|
Tube |
Distilled Water |
Catechol
|
PTU (Inhibitor) |
Potato Extract |
Results (Absorbance)
|
|
1 |
5.5 ml |
0.5 ml |
-- |
--- |
0.02 |
|
2 |
5 ml |
0.5 ml |
--- |
0.5 ml |
0.68 |
|
3 |
4.5 ml |
0.5 ml |
0.5 ml |
0.5 ml |
0.04 |
|
4 |
4.5 ml |
0.5 ml |
|
1.0 ml |
1.26 |
|
5 |
4.5 ml |
1.0 ml |
|
0.5 ml |
1.22 |
a) Explain with respect to enzyme activity why test tube 1 produced very little absorbance:
b) Explain with respect to enzyme activity why test tube 3 produced very little absorbance:
c) Explain how to tubes 4 and 5 differ with respect to reaction conditions.
d) Explain with respect to enzyme activity why tubes 4 and 5 have similar absorbance and why both have greater absorbance values than tube 2.
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