Use the Stokes-Einstein equation to estimate the diffusion coefficient in m²/s and in µm²/s for the following particles. Assume that the particles are immersed in water in a test tube at body temperature (37 °C), which has a viscosity of approximately 0.0007 kg m ¹ s¹. Assume the particles are all spherical Particle ATP Eukaryotic ribosome SARS-CoV-2 Diameter 1.4 nm 25 nm 100 nm
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- Calculate the effective quantity (g) of sodium chloride related to tonicity in 100 mL of an intravenous fluid labeled "5% dextrose in 0.45% sodium chloride," and indicate whether the solution is isotonic, hypotonic, or hypertonic.The potato blocks used in this experiment are rich in starch molecules. Iodine potassium iodide (IKI) is a starch indicator that forms a blue-black complex in the presence of starch. What does the width of the black of the potato cube represent in the context of diffusion? How does the width of the black iodide starch complex of each potato block compare to the others? Why? Table 1a Why Are Cells Small Length (l) (cm) Width (w) (cm) Height (h) (cm) Size of Cross Section (h x w) (cm) Distance Traveled by IKI from Potato Edge (cm) Area of White Region (l x w) (cm) 2.50 2.50 2.50 6.25 cm 0.20 cm 2.64 cm 2.00 2.00 1.00 2.00cm 0.30 cm 1.87 cm 1.50 1.50 1.50 2.25 cm 0.50 cm 1.50 cm 1.00 1.00 1.00 1.00 cm 0.40 0.60 cm 2.00 0.50 0.50 0.25 cm 0.20 cm 0.15 cm 0.50 0.50 0.50 0.25 cm 0.30 cm 0.10 cmYou have 3 cubes, A, B, and C. They have surface to volume ratios of 3:1, 5:2, and 4:1 respectively. Which of these cubes is going to be the most effective at maximizing diffusion, how do you know that?
- Estimate the TKN associated with a sample having 50 mg/L of cell tissue and 10 mg/L of ammonia. Assume cell tissue has a molecular composition of C5H7O2N.The average time it takes for a molecule to diffuse adistance of x cm is given byt = x2/2D where t is the time in seconds and D is the diffusioncoefficient. Given that the diffusion coefficient ofglucose is 5.7 × 10−7cm2/s, calculate the time it wouldtake for a glucose molecule to diffuse 10 μm, which isroughly the size of a cell.If the osmotic pressure of a 7.96×10-2-M aqueous solution of Fe2(SO4)3 was found to be 8.71 atm at 20°C, what would be the "observed" van't Hoff factor? Use the above van't Hoff factor to predict the freezing point of this solution.Note that the corresponding molality would be 8.25×10-2 m, given that the density of the solution is 0.997 g/cm3. °C Use the above van't Hoff factor to predict by how many degrees the boiling point of this solution will be elevated above that for pure water.
- How is the rate of diffusion affected by an increasedconcentration gradient? By increased temperature of asolution? By increased viscosity of the solvent?If you made a hematocrit in two tubes. Tube A is made of 0.60M NaCl, and Tube B is made of 1.00M of NaCl, describe how the cell would respond in these solutions? Which would have a higher rate of osmosis? If the cell is permeable to NaCl, which would have a higher rate of diffusion?We have isolated a mixture of 2 protein of the same molecular weight but different isoelectric points. The isoelectric points are 7.5 for Protein A and 9.0 for Protein B. What would be the order of elution from a cation exchange column if a buffer of pH 7 is used? Group of answer choices Both proteins will remain in the column. Both proteins will elute simultaneously Protein B followed by Protein A Protein A followed by Protein B
- What is the osmotic pressure of a 38 M glucose solution that intravenously injected to the blood at 37 degrees Celsius?Determine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis.The isoelectric point of eIF4a is 5.02. Students are given a sample of cell lysate containing eIF4A at pH 7.4. They are also provided with two buffers of pH 7.4 to use for ion exchange chromatography. One buffer has a very high salt concentration (1 M), the other has a low salt concentration (0.1 M). Mariela decides to use an anion exchange column, while Ashok chooses a cation exchange column. Who made the better decision? Provide a detailed explanation of why one student will end up with a purer sample of eIF4A. Which buffer will you use while washing away impurities (high or low salt), and which would you use to remove eIF4A from the column? Explain your choice.