Using the concept of gene therapy describe how you can design a vehicle to target SARS-CoV-2 RNA?

Q: (A)What strategy/type of vaccine would you pursue with the hopes of developing a vaccine to a new…

A: *Coronaviruses large and enveloped RNA viruses containing single stranded RNA positive sense strand.…

Q: Write five applications of CRISPR/Cas system in diagnosis and therapy with examples

A: CRISPR-Cas9 is genome editing tool that enables geneticists medical researchers to edit parts of the…

Q: Compare and contrast the roles of crRNA and tracrRNA in the defense process against bacteriophages…

A: Cas9 in conjunction with the engineered CRISPR sequences can be used to hunt down the codes and them…

Q: a. deleting that protein from an organism (knock-out).

A: Antisense can be defined as the strand of DNA that is not involved in coding. There are several…

Q: How do 3D protein modeling tools help in the development of drugs or vaccine?

A: The 3D structures of the desired target proteins, drugs and epitopes can be predicted and modeled…

Q: Describe the steps that lead to the formation of new HIVparticles.

A: The human immunodeficiency virus (HIV) is an ribonucleic acid (RNA) virus that attacks the immune…

Q: which human cell machinery do the SARS-CoV-2 uses in order to replicate?.

A: The current COVID-19 pandemic is due to the novel coronavirus SARS-CoV-2.

Q: gene therapy

A: It may or may not be. But right now gene therapy to not eliminate Covid 19.

Q: Explain how viral vectors can be used for gene therapy, and provide two examples of commonly used…

A: The two methods of gene transfer used in gene therapy are: The viral-mediated gene transfer…

Q: In the SARS-CoV-2 assay, what is the benefit of a diagnostic tool that can distinguish the…

A: The novel Coronavirus infection SARS CoV 2 can be diagnosed by different means. Proper diagnosis,…

Q: hat are some of the challenges of using recombinant molecules to treatment of HIV?

A: HIV (human immunodeficiency virus) affects immune cells that aid in the body's ability to fight…

Q: What are the two type of viral vector involved in gene therapy trials?

A: Viral vectors are the engineered tools which are used by molecular biologists to transfer genetic…

Q: plain the fundamental difference between CRISPR/Cas9 and in vivo adenovirus-based gene therapy

A: All biological data of AN organism is held on within the biomolecule known as DNA. gene is the…

Q: Describe how a plaque assay is used to detect and estimate the numbers of phage particles.

A: Plaque is a term used in microbiology for a clear zone on a bacterial culture plate that indicates…

Q: What is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

A: Gene therapy is a method that allows the correction of the defective gene. In gene therapy, it is…

Q: Using PEN and PAPER ONLY, illustrate a SARS-CoV-2 genome organization showing all the genes. Label…

A: SARS-CoV 2 is a virus that affects several organs in humans like the heart, kidneys, and very…

Q: Explain the main defects of using animal models for viral growth and cultivation.

A: Viruses are highly infectious organisms that cannot be cultivated in non-living culture media. They…

Q: Two approaches for correcting single-gene defects are gene therapy such as is discussed in Section…

A: Bacteria are microscopic organisms that positioned in prokaryote as these are unicellular organisms.…

Q: What distinguishes a retrovirus from an LTR transposon, and how is this difference enabled?

A: LTR retrotransposons are class I transposable elements characterized by the presence of long…

Q: escribe which human cell machinery the SARS-CoV-2 uses in order to rep

A: Some useful terms :  SARS ( Severe acute respiratory syndrome), CoV ( covid 19)  ACE 2 (…

Q: Transmission studies suggest that there are at least three (3) different variants of the SARS-CoV-2…

A: SARS-CoV2 is a virus consisting of four main structural proteins called spike, membrane, envelop,…

Q: Describe the three steps of CRISPR-Cas–mediated antiviral defense.

A: CRISPR stands for Clustered regularly interspaced short palindromic repeats and Cas stands for their…

Q: Describe the role of the Gag polyprotein during the assembly of HIV components at the host-cell…

A:

Q: Explain the role of RNase H (a component of reverse transcriptase)during the synthesis of HIV DNA.

A:

Q: What are some of the major human enzymes, structure, and proteins needed for SARS-CoV-2 replication…

A: The family of positive-sense, single-stranded RNA-enclosed viruses known as coronaviruses (CoVs) is…

Q: Find out if BamHI is a good restriction endonuclease for cutting the SARS-COV-2 genome? Give…

A: Endonucleases are enzymes that cut within the sequence of the DNA. Restriction endonucleases…

Q: Describe how we can use genetic techniques to identify the presence of viral genetic information in…

A: Genetic techniques are those laboratory/diagnostic techniques which involve any gene or gene product…

Q: You receive purified mRNA encoding the peak protein of SARS Cov-2 as well as the plasmid pET32 (a…

A: DNA cloning with plasmid vector involves the addition of a foreign gene into a plasmid vector. The…

Q: . a. Compare the means by which retroviral and AAVvectors deliver therapeutic genes to human…

A: The viruses are considered to be non-living when they are out of the host as they lack cellular…

Q: Describe any three vectors less method of introducing the rDNA into a competent host cell?

A: rDNA is the recombinant DNA which is formed by combining desired genes from different organisms. It…

Q: Write in detail about the effects of presence as well as absence of MCS and reporter gene in a…

A: MCS (Multiple Cloning Sites ) -- Multiple cloning site is region of D.N.A. with in a plasmid that…

Q: Discuss the similarities and differences. How can you use this information to design therapy for…

A: Gene regulation is the procedure of controlling which genes in a cell's DNA are expressed (used to…

Q: using the concept of gene therapy,describe how to design a vehicle to target SARS CoV-2 RNA?

A: Gene therapy is used a gene delivery system to insert a gene into the nucleus of the cell for the…

Q: Explain a ideal molecular surfactant design for mRNA vaccines

A: Answer :- mRNA vaccine address a promising option in contrast to regular immunization approaches in…

Q: Write the advantages and disadvantages of applying such application in the DNA of an organism. 2.…

A: According to the question, we have to write down the advantages and disadvantages of applying such…

Q: How could sequencing many SARS-CoV-2 genomes be used to track how the virus has changed over time…

A: Mutation It refers to the alteration of the sequence of the DNA or RNA in organisms. These mutations…

Q: Does CRISPR CAS9 as a defense in bacteria only work for double stranded viral DNA? or any type of…

A: Scientists can use CRISPR to persistently edit genes in living cells and creatures and, in the…

Q: explain the full method you would use to sequence the full genome of SARS-Cov-2

A: The development of SARS-CoV-2, which causes COVID-19 illness, has generated a massive worldwide…

Q: Describe two methods for transforming a vector into bacteria.

A: Transformation Transformation is the transfer of genetic information through the agency of free DNA…

Q: Discuss how recombinant DNA technology could be used to develop a control strategy for the novel…

A: The SARS CoV2 has propensity for upper respiratory tract. The spike protein of SARSCoV2 binds to…

Q: Suppose you transformed a plasmid vector carrying a human interferon gene into E. coli but none of…

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: Briefly explain the role of arabinose in the expression of the GFP gene of the pGLO plasmid.

A: Answer: Introduction: pGLO plasmid have DNA sequences which allow its replication and expression of…

Q: Discuss retroregulation and its significance in the biology of bacteriophage lambda during: a)…

A: Introduction Retroregulation can be defined as the control of gene expression from sites distal to…

Q: Viral gene delivery vectors are favored for therapeutic applications because of their efficiency and…

A: Viral gene delivery vectors or the viral vectors are the most preferred method of gene transduction…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.