In the SARS-CoV-2 assay, what is the benefit of a diagnostic tool that can distinguish the difference between the presence of IgG and IgM in a sample?
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In the SARS-CoV-2 assay, what is the benefit of a diagnostic tool that can distinguish the difference between the presence of IgG and IgM in a sample?
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- If you wanted to make an assay detecting SARS-CoV-2 virus presence, like the assay shown in the previous question, what would be a logical combination of things to include in your list of supplies to obtain? rabbit anti-SARS-cov2 spike antibody, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike antibody, anti-mouse horseradish peroxidase conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike antibody, anti-rabbit SARS-cov2 spike antibody, tetramethylbenzidine SARS-cov2 spike, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike horseradish peroxidase conjugated antibody, anti-rabbit horseradish peroxidase conjugated antibody, tetramethylbenzidineIf antibody responses are not elicited by a Covid-19 vaccine, are there other types of immune response that could provide protection from the SARS-Co-V2 virus following vaccination? Explain why or why not.Using PEN and PAPER ONLY, illustrate a SARS-CoV-2 genome organization showing all the genes.Label the 4 structural genes and the 2 genes that code for the PP1a and PP1b and produces 16nonstructural proteins.
- please help explain would SARS-CoV-2 N-nucleocasid protein be good target for neutralizing antibodies and vaccine development ?and whyIn ELISPOT Assay, explain the importance of adding stimulus to the 96-well plate during the incubation of Peripheral Blood mononuclear cellswhich human cell machinery do the SARS-CoV-2 uses in order to replicate?.
- What is the purpose of anti-human IgG antibodies in immunological testing?In a mixed infection assay between h+r+ and hr phage, where h and r are two genes that govern plaque morphology, which of the following do you expect to observe? only h+r+ and hr only h+r and hr+ only h+r+ only hr All four types: h+r+, hr, h+r, hr+Which of the IgG subclasses would you think was in principle most desirable for use as a therapeutic monoclonal antibody, and why? Are there any disadvantages to using this subclass and how might they be overcome?
- In the plaque assay, what is the precise origin of a single plaque?From the MW analysis; are any of the gel and immune-detected bands consistent with what you expect for IgG? Are the gel and immune-detected bands suspected to be IgG consistent in distance migrated with to with respect to the molecular ladder? What is the difference between the whole calf serum and the “purified” lanes? Were we able to truly purify IgG with the magnetic bead protocol?What are some ways that the antibody-protein complex can be visualized in ELISA or western blots?