Using the TABLE shown, identify which proteins require pre-treatment with performic acid before amino acid sequencing. 1 & 5 2 & 4 3, 4 & 5 1 & 2

Q: What is the point of hydrolyzing RNA before doing biochemical tests?

A: Asked: The reason of hydrolyzing the RNA before doing biochemical tests.

Q: Explain Peptide Mapping and Internal Sequencing of Proteins from Acrylamide Gels.

A: Peptide mapping is an identity test for proteins, those obtained by rDNA technology. It involves the…

Q: Are all the proteins separated properly using SDS-PAGE? Why or why not?

A: Proteins are massive biomolecules and macromolecules made up of one or more long chains of amino…

Q: In assessing extracted DNA quality, what does A280 indicate? aromatic amino acids nitrogenous bases…

A: DNA is extracted from specific cells to study the extracted DNA. The purity of extracted DNA is…

Q: What is the importance of the 6.25 multiplication factor for protein analysis (Kjeldahl methods)?

A: The Kjeldahl method for protein measurement was designed to be used for the measurement of nitrogen…

Q: Name the technique used to separate fragments of DNA or mixtures of proteins?

A: The gel electrophoresis is a technique to separate DNA fragments and proteins based on their size,…

Q: Explain the origin of the signal that is used in the nucleic acid sequencing method known as…

A: Ion torrent technology is a modern genome sequencing method that converts chemically encoded…

Q: How do you verify amino acid sequences with proteins and species with BLAST?

A: Bioinformatics is an interdisciplinary field of study which has diverse application in the…

Q: Briefly describe the importance of two dimensional electrophoresis in protein separation?

A: Two-dimensional gel electrophoresis is the technique that is used in the separation of the proteins…

Q: List all the components and explain their purpose in the reaction mixture used for the dideoxy DNA…

A: The method of evaluating the sequence of nucleotides (As, Ts, Cs, and Gs) in a portion of DNA is DNA…

Q: Approximately how large is the Escherichia coli genome in basepairs? How many genes does it contain?

A: Escherichia coli also known as E. coli is a gram negative, facultative anaerobic, rod shaped,…

Q: What types of homogenization techniques are available for solubilizing a protein?

A: In the process of slating out, the solubility of the protein reduces due to the highly ionic salt…

Q: steps the Edman degration for amino acid sequencing both the N- and C- terminal analysis

A: Edman degradation is a method for sequencing of amino acids using specific biochemical compounds and…

Q: What would happen during an amino acid sequencing experiment using the Edman degradation if you…

A: The sequence of amino acids can be determined by using amino acids which are present in peptide and…

Q: pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI:…

A: Given: Digestion of a plasmid PMDawn with EcoRI, BamHI, and EcoRI+BamHI are as follows: EcoRI: 20 kb…

Q: The following list of proteins are found in a mixture and are to be separated by 2D gel…

A: In 2D gel electrophoresis, is a 2 step-based separation of protein.  In the first dimension, the…

Q: Complete the DNA and RNA sequencing for the translation and creation of proteins. 1. DNA  3' CCC…

A: The genomic structure of an organism consists of DNA which contains sugar molecule, phosphate group,…

Q: What special kind of nucleotide is used in the Sanger sequencing procedure?

A: A British biochemist named Fred Sanger and few of his research colleagues developed the process of…

Q: Fill in the purity reading of each either good or bad sample as well as the remarks what makes the…

A: There are few important points: RNA is synthesized by four types of ribonucleotides and joined by…

Q: In the procedure called RNA sequencing (RNA-Seq), what type ofmolecule is actually sequenced?

A: Ribonucleic acid sequencing (RNA-seq) was developed by Michael Snyder and colleagues in 2008. It is…

Q: What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis…

A: DNA is a hereditary molecule found in cells that transmits genetic information from one generation…

Q: Describe how proteins are sequenced, and explain why sequence determination is important

A: Protein sequencing is the technique to determine the amino acid sequence of a protein. Proteins are…

Q: Why is it necessary to load the same amount of protein for each sample during electrophoresis?

A: Electrophoresis can be described as the movement of particles (charged particles) in a gel or fluid.…

Q: Use the sequence alignment tool Smith and Waterman for the amino acid number 16-30 of the protein…

A: The Smith-Waterman algorithm uses local sequence alignment to find similar regions between two…

Q: pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI:…

A: Agarose gel electrophoresis is a method of gel electrophoresis that is used to separate a mixed…

Q: Can DNA be isolated from beef? Discuss the process briefly

A: The purification of DNA from the collected sample of DNA with the help of the various methods and…

Q: Explain the importance of adding TE Buffer as the final solution to isolate the concentrated DNA.

A: DNA isolation is a process of extracting nuclear material from a tissue sample. The tissue sample is…

Q: IT IS POSSIBLE TO ESTIMATE THE CONCENTRATION OF SPECIFIC DNA SEQUENCE IN A SOLUTION OF MIXED DNA.…

A: DNA can be quantified through nanodrop with a particular absorbance.

Q: The oligonucleotide d-ATGCCTGACT was subjected to sequencing by Sanger’s dideoxy method, and the…

A: Electrophoresis is a technique that separates the molecules basing on the differences in the charges…

Q: What are two ways to denature a protein and why do these methods work?

A: Given:  Two ways to denature a protein.

Q: In ion torrent DNA sequencing, covalent bond formation is detected by an ion sensor sensitive to…

A: It's a DNA sequencing method

Q: What is the purpose of the dideoxynucleotide triphosphates in the Sanger sequencing reaction?

A: In 1977, Sanger proposed chain termination method of DNA sequencing. He used ddNTP or…

Q: Discuss the underlying biochemical principle of the nucleic acid sequencing methods known as…

A: One of the techniques for figuring out the sequence of DNA or RNA is called next-generation…

Q: What are some common protein identification techniques?

A: Proteome: The set of all expressed proteins in a cell, tissue, or organism. Proteomics: A science…

Q: Using Bradford Assay, plot the standard curve and find the unknowns. Protein Concentration A595 of…

A: Standard curve  A set of standards are put up in the Bradford assay procedure from protein sources…

Q: Transcribe the following DNA nucleotide sequence and determine their respective amino acid products.…

A: Transcription  Formation of RNA over DNA template is called transcription.

Q: how would you identify whether or not complete resolution of the three amino acids has been achieved

A: An amino group and an acid group-containing organic molecules are called Amino acids. At the point…

Q: Why was an SDS-PAGE gel used when working with a protein as opposed to an agarose gel? What dye was…

A: SDS PAGE, abbreviation of Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis is a laboratory…

Q: Sanger sequencing originally used 4 lanes in gels. These lanes represented sequences of different…

A: Sanger sequencing is a method of DNA sequencing based on the selective incorporation of…

Q: Suppose that there is a protein consisting of two polypeptide chains with the given sequences in the…

A: Edman degradation, which was invented by Pehr Edman, can be used to determine the amino acid…

Q: Discuss the main similarities and differences in the nucleic acid sequencing methods known as…

A: DNA sequencing is the process of determining the sequence of nucleic acids, which are the basic…

Q: Explain how to prepare 2 ml of a solution with a concentration of 1 μg/5ml from a stock solution of…

A: Given- Stock solution of DNA sample- 0.1 mg/ml Volume required= 2 ml Concentration of the DNA stock…

Q: What are the various techniques in Molecular Biology used for the Isolation, purification, and…

A: Introduction Proteins:- A protein is an extremely complex natural substance made up of amino acid…

Q: On the image below what is the next nucleotide to be added on the oldest Okazaki fragment (use…

A: Answer- The next nucleotide to be added on the oldest Okazaki fragment will be in the direction 5'…

Q: How is the Bicinchoninic acid assay used in protein quantification?

A: The Bicinchoninic Acid Assay (BCA), often known as the Smiths' Assay, was developed by Paul Smith in…

Q: Amino acid compositions can be determined by heating a protein in 6 M HCl and running the…

A: Introduction: An impure protein sample is stacked into the IE column at a specific pH. Charged…

Q: How is PAGE (Polyacrylamide Gel Electrophoresis) different from other protein  purification…

A: Protein purification is a process of isolation of specific protein from the complex protein mixture…

Q: Which fractionation procedures separate nucleic acids according to their binding affi nity?…

A: Nucleic acids are the major biomolecules essential for life. It includes DNA(Deoxyribo nucleic acid)…

Nutrition and diet

We are involved in several daily activities. These actions need a significant quantity of energy. The food we eat provides this energy. Food is necessary for development, repair, and other living activities since it supplies energy. All of these are part of the living practice known as nutrition. Nutrition is the study of nutrients in food, how the body uses them, and the relationship between diet, health, and disease. Nutritionists employ concepts from molecular biology, biochemistry, and genetics to understand how nutrients affect the human body. Nutrition also looks at how people can use their diet to reduce their risk of disease, as well as what happens when they ingest too much or too little of a nutrient and how allergies emerge.