The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis: Protein Molecular weight (Daltons) Isoelectric point Myoglobin 17,000 7.0 Cytochrome C 12,000 10.6 IgG 150,000 6.4 Hemoglobin 64,500 7.1 Insulin 5,800 5.4 Human serum albumin 66,000 4.6 Histone H3 15,000 10.8 Pepsin 33,000 1.0 In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller proteins go towards the bottom. 2 4 pH 7 10 direction of electric current 150 MW (kDa) 100 В E 50 2 4 pH 7 10 With this information, match the letters of the bands to the proper protein based on the data given. Band Letter Protein A В E F G H
The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis: Protein Molecular weight (Daltons) Isoelectric point Myoglobin 17,000 7.0 Cytochrome C 12,000 10.6 IgG 150,000 6.4 Hemoglobin 64,500 7.1 Insulin 5,800 5.4 Human serum albumin 66,000 4.6 Histone H3 15,000 10.8 Pepsin 33,000 1.0 In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller proteins go towards the bottom. 2 4 pH 7 10 direction of electric current 150 MW (kDa) 100 В E 50 2 4 pH 7 10 With this information, match the letters of the bands to the proper protein based on the data given. Band Letter Protein A В E F G H
Chapter1: Relative Value, Addition, And Subtraction Of Decimals
Section: Chapter Questions
Problem 27SST
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Transcribed Image Text:The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis:
Molecular weight (Daltons)
Protein
Isoelectric point
Myoglobin
17,000
7.0
Cytochrome C
12,000
10.6
IgG
150,000
6.4
Hemoglobin
64,500
7.1
Insulin
5,800
5.4
Human serum albumin
66,000
4.6
Histone H3
15,000
10.8
Рepsin
33,000
1.0
In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their
isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via
molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller
proteins go towards the bottom.
2
4
pH
7
10
direction of electric current
150
MW (kDa)
100
В
E
A
50
2
4
pH
7
10
With this information, match the letters of the bands to the proper protein based on the data given.
Band Letter
Protein
A
D
E
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