VSV-GFP- SARS-CoV VSV-GFP- SARS-CoV-2 U Plaque Day 3 (duplicate) 1.51 1.0- 0.5- 0.0 VSV-GFP- SARS-CoV VSV-GFP- SARS-CoV-2 VSV-GFP- VSV-GFP- SARS-CoV SARS-CoV-2 Fig. 2. rVSV expressing SARS-CoV-2 spike spreads faster than does rVSV bearing SARS-CoV spike. Vero-E6 cells were infected with rVSV-GFP-SARS-CoV-2 or SARS-CoV (MOI = 0.01); 1 h postinfection, cells were washed with PBS and cultured in the presence of 1% methylcellulose. Photos were taken at 18 h and 24 h (A). After 72 h of infection, cells were fixed with 3.7% paraformaldehyde (PFA) and stained with crystal violet (B). The number and size of pla- ques are plotted in C and D, respectively. ***P<0.001. ns, not significant. Plaque number 80 60 40 20 18 h (10X) ns 24 h (20X) B D Plaque size (mm)

VSV-GFP- SARS-CoV VSV-GFP- SARS-CoV-2 U Plaque Day 3 (duplicate) 1.51 1.0- 0.5- 0.0 VSV-GFP- SARS-CoV VSV-GFP- SARS-CoV-2 VSV-GFP- VSV-GFP- SARS-CoV SARS-CoV-2 Fig. 2. rVSV expressing SARS-CoV-2 spike spreads faster than does rVSV bearing SARS-CoV spike. Vero-E6 cells were infected with rVSV-GFP-SARS-CoV-2 or SARS-CoV (MOI = 0.01); 1 h postinfection, cells were washed with PBS and cultured in the presence of 1% methylcellulose. Photos were taken at 18 h and 24 h (A). After 72 h of infection, cells were fixed with 3.7% paraformaldehyde (PFA) and stained with crystal violet (B). The number and size of pla- ques are plotted in C and D, respectively. ***P<0.001. ns, not significant. Plaque number 80 60 40 20 18 h (10X) ns 24 h (20X) B D Plaque size (mm)

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.10: Principles Of Automated Hematology

Problem 2CS

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