please answer g 3. The main components of clove oil are eugenol, eugenol acetate, and b-caryophyllene.ноeugenoleugeoal acetateB-caryophyllenea) Rank the compounds from least polar to most polarb) After analyzing clove oil by TLC using a 10% acetone in hexanes mobile phase, you obtain the followingTLC results. Identify the compound that corresponds with each spot.5 cm2,8 cmc) Calculate the Rr value of the middle spot. Show your workd) What would happen to the Rr of the spots if you increased the polarity of the mobile phase? Explaine) When separating the compounds by column chromatography, which compound would you expect to elutefirst? Which compound would you expect to elute last?f) You perform column chromatography on a sample of clove oil and collect a total of 16 fractions. Youanalyze each fraction by TLC using four TLC plates. One plate has fractions 1-4, one has fractions 5-8,one has fractions 9-12, and one has fractions 13-16. After developing all of your TLC plates, however, yourealize that you forgot to label the TLC plates with the fraction numbers. Label the TLC plates below withthe fraction that they contain (1-4, 5-8, 9-12, 13-16).-+-F-F-F---+------++-F-F--++-F-F-g) Was your column chromatography separation of the three compounds in clove oil successful? Explain whyor why not.

Answered: Was your column chromatography…

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Was your column chromatography separation of the three compounds in clove oil successful? Explain why or why not.

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter33: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 33.15QAP

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

please answer g

3. The main components of clove oil are eugenol, eugenol acetate, and b-caryophyllene.
но
eugenol
eugeoal acetate
B-caryophyllene
a) Rank the compounds from least polar to most polar
b) After analyzing clove oil by TLC using a 10% acetone in hexanes mobile phase, you obtain the following
TLC results. Identify the compound that corresponds with each spot.
5 cm
2,8 cm
c) Calculate the Rr value of the middle spot. Show your work
d) What would happen to the Rr of the spots if you increased the polarity of the mobile phase? Explain
e) When separating the compounds by column chromatography, which compound would you expect to elute
first? Which compound would you expect to elute last?
f) You perform column chromatography on a sample of clove oil and collect a total of 16 fractions. You
analyze each fraction by TLC using four TLC plates. One plate has fractions 1-4, one has fractions 5-8,
one has fractions 9-12, and one has fractions 13-16. After developing all of your TLC plates, however, you
realize that you forgot to label the TLC plates with the fraction numbers. Label the TLC plates below with
the fraction that they contain (1-4, 5-8, 9-12, 13-16).
-+-F-F-F-
--+------
++-F-F-
-++-F-F-
g) Was your column chromatography separation of the three compounds in clove oil successful? Explain why
or why not.

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