3. Compound A has an Rf of 0.75 and compound B has an Rf of 0.35. Which compound will elute first from a silica gel column using the same solvent system as in TLC?
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- How does increasing the polarity of the developing solvent affect the Rf of a compound on a silica gel TLC plate?Why mixture of solvents such as ethyl acetate with 5% acetic acid is used as a solvent/mobile phase for developing TLC? Please explain in detail.Doe hydrophobic silica gel act as non-polar stationary phase in TLC making the process as reverse chromatography? explain
- Explain why it is needed to filter the solvent before introducing it to the HPLC systemExplain how solid-phase microextraction works. Why is cold trapping necessary during injection with this technique? Is all the analyte in an unknown extracted into the fiber in solid-phase microextraction?List 3 criteria that is important to consider when choosing a recrystallization solvent In a TLC analysis, a student find that all his spots have an Rf value less than 0.05. What should the student do to achieve better separation?
- How might the Rf values of colorless substances be determined? That is, how could the colorless spots be located? (for paper chromatography)Compound A has a lower affinity for the stationary phase thancompound B and can dissolve well in the mobile phase. In theseparation of the mixture of A and B by column chromatography,which compound will be eluted first from the column? ExplainTwo compounds A and B from GC on two open tubular columns contains a XY-1 (100% methyl) and a XY-2 (50% methyl, 50% phenyl), with the same diameter, thickness and length under the same experimental conditions. The retention times and peak widths are show in the Table 1.Hint: phenyl groups interact more strongly with polar groups than methyl groups. Which compound is more polar? Explain your answer
- Thin layer chromatography experiment. Show calculations for the Rf of each spot you observed.Which is more polar, the water solvent or the chromatogrphy paper?Thin Layer Chromatography why the non-polar compounds move up the plate most rapidly (higher Rf value), whereas polar substances travel up the TLC plate slowly or not at all (lower Rf value)? Please help me explain this question! Thank you so much!