What are cells that have been made ready for transformation by exposure to chemicals called? O chemically competent O electrocompetent O universally competent O field competent
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- QUESTION 27 Which of the following methods can be used to compare the amounts of one specific mRNA that is expressed by two different cell lines? A. Immunohistochemistry B. Western blotting C. Polymerase chain reaction (PCR) D. ImmunocytochemistryQuestion 9 What is the most likely reason that there were so few bacterial colonies growing on the streptomycin-positive plate in the first part of the experiment? 1. Streptomycin in the medium caused deleterious mutations in the bacteria. 2. Only a few individuals in the original bacterial culture had the mutation that conferred resistance to streptomycin .Nanotechnology Devices can improve human health. But can they do harm or be abused? What regulations would allow for beneficial uses of nanorobots without allowing for harm?
- Question 2. After sterility test of an injectable it was observed that an injection is failed the test what will you do then depending on the product nature (heat sensitive/ heat resistant) (Not more than one sentence)? Mention the purpose of product inhibition test and how it is performed (Not more than one sentence) ? How will you check the validity of a medium (Not more than one sentence) ?Question 12 Imagine you are a cytogeneticist preparing a karyotype, but you forgot to add the colchicine in the early part of the protocol. You are not aware of the mistake, until you look at the slides, as you are getting ready to photograph the slides of the cells that have been dropped, fixed, and stained. What would you see on the slides, that would tell you that you made a mistake earlier in the karyotype protocol?Question 3Which of the following statements regarding conjugation pili is correct? Question 3 options: a) They facilitate the transfer of RNA between bacterial cells b) They contribute to antibiotic resistance c) They are involved in sexual reproduction d) They are longer than fimbriae and flagella
- QUESTION 4: Why does a PCR reaction require a primer? Would you expect this primer to be composed of DNA or RNA? Explain your reasoning.Question 5 You spread a small amount of a water sample from the environment on an enriched media agar plate. After 24 hours, the plate is covered with many small yellow colonies. Does this plate contain a pure culture of bacteria? ○ No, because it came from the environment, there are lots of different organisms. ○ We know there are multiple species present, but each colony is a different species. ○ Yes, we know the plate contains a pure culture because there are individual colonies. ○ We do not know if the plate contains a pure culture or a mixed culture. or even if individual colonies are pure or mixed based on their appearance alone.Question #2 To isolate DNA from cells, you must disrupt the cell Therefore, you treated your cells with a lysis buffer that contains a detergent. You also heated the cells and vortexed them during this time. What chemical property does the detergent in the lysis buffer have that helps disrupt a cell membrane? Briefly explain your answer. How does vortexing and heating your samples aid in disrupting the membrane?
- Question 1: Which of the following processes is not involved in genetic engineering? A. DNA polymerases are used to seal the bonds between fragments B. reinserting DNA into living organisms C. recombinant plasmid gets inside a bacterial cell D. cutting out a DNA sequence E. changing a DNA sequence Question 2: In genetic engineering, vectors are needed in the process and play a crucial role because these molecules A. are able to covalently bond and carry foreign DNA into cells B. protect host cells from invasion by foreign DNA C. degrade nucleic acids D. help in replicationQuestion 15 How did you select and grow a streptomycin resistant strain of bacteria in this experiment? 1. A sample of bacteria was exposed to streptomycin by inoculating it onto a streptomycin negative plate. Colonies that grew carried a mutation for resistance 2. A colony of bacteria was with streptomycin so that the antibiotic could alter then genetic composition of the bacteria . 3. A sample of bacteria was exposed to streptomycin by inoculating it onto a streptomycin positive plate. Colonies that grew carried a mutation for resistanceQuestion 15 How did you select and grow a streptomycin resistant strain of bacteria in this experiment? 1. A sample of bacteria was exposed to streptomycin by inoculating it onto a streptomycin negative plate. Colonies that grew carried a mutation for resistance 2. A colony of bacteria was with streptomycin so that the antibiotic could alter then genetic composition of the bacteria . 3. A sample of bacteria was exposed to streptomycin by inoculating it onto a streptomycin positive plate. Colonies that grew carried a mutation for resistance 4. Samples of bacteria were taken from the culture and observed under a microscope for signs of susceptibility or resistance . Those that were resistant were separated and plated .