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What are the advantages and disadvantages of plate count technique over other methods of quantifying microbial populations?
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- what criteria that a microorganism must meet before it can be considered an “indicator?”What are the disadvantages of doing a total microbial count by examining wet mounts under the microscope?What is the advantage of using Molecular data compared to plated culture or biochemical techniques in identifying bacterial species, especially for oral microbes ?
- What is the difference between a mixed culture and a contaminated culture if they both contain more than one type of organism ?A microbiologist used the concept of enrichment culture to isolate aerobic and anaerobic nitrogen-fixing bacteria, sulfate-reducing bacteria, and sulfur-oxidizing bacteria. What kind of selective media could he haveused for isolating each of these four classes of microbes?What are the potential consequences of long-term and widespread use of antimicrobial therapy on microbial populations and their evolution?
- You have learned many different techniques so far this semester that allow us to identify pathogenic microbes in the clinical setting. Why do you think rapid methods are so helpful? Why are traditional tests such as metabolic tests not always used to ID clinically important microbes?What are challenges or difficulties we may encounter for identifying a bacillus cereus microbe ?What is the answer of microbial load in the culture?