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- 1. Explain the regulation of microbial growth by turgor pressure.http://vlab.amrita.edu/?sub=3&brch=73&sim=1628&cnt=1 Explain how the Kirby-Bauer method relies on diffusion of antibioticsSuppose you spilled two cultures of Salmonella typhimur-ium (each containing 100,000 cells) on your lab bench. You im-mediately applied the same disinfectant to both cultures at thesame time. One culture had been freshly grown for 36 hours andthe other culture is 2 weeks old. If the kill ing rate of the disin-fectant is 90% per minute, do you think that microbes in bothcultures will be completely killed after 6 minutes? Why or whynot?
- Identify the process orenvironment in this list that is notaffected by microorganisms.a. oxygen cyclesb. global temperaturesc. human healthd. all of the above have microbialinvolvementEvery year, supposedly safe municipal water supplies causeoutbreaks of enteric illness.a. How in the course of water analysis and treatment might thesepathogens be missed?b. Why is there less tolerance for a fecal coliform in drinking orrecreational water than for other bacteria?Are natural compounds such as cellulose and petroleum oil easier for microorganisms (eg. bacteria etc.) to degrade than man-made compounds such as plastics?
- Chopped garlic often contains C. botulinum endospores and iscommonly sold submerged in olive oil, which creates an anaerobicenvironment. What is required to be added to garlic sold in thismanner?a. sodium chlorideb. citric acidc. waterd. A warning label describing the symptoms of botulism.A microbe is only capable of growth at pH range of 8.5-10 in the presence of atmospheric levels of oxygen. Which statements are correct? Mark all that apply. A.)The microbe is likely to be a human pathogen B.)The microbe is a mesophile C.)The microbe is an obligate aerobe D.) The microbe is an alkiliphile1. “Coliform bacteria are the only group of micro-organisms considered suitable for themonitoring of faecal pollution.”Is the statement above still valid? Motivate your answer and briefly describe at two othertypes of indicator organisms. 2.
- TSA plates of microorganisms grown at 30°C or 37°C In words, describe the differences you see for each organism (E. coli M.,luteus ,S. marcescens and S. saprophyticus) grown at 30°C or 37°C (ie, amount of growth, color, etcWhi discovered an antibiotic when returning from vacation in Scotland and found his bacterial samples were contaminatedA food sample with an unknown number of contaminating bacteria was serially diluted to 10-6. A volume of 0.1 mL (100 μL) of the dilutions was plated. The 10-6 plate contained 96 colonies. What was the original microbial concentration?