What are the advantages of real-time PCR over microscopy for diagnosing malaria? a. Giemsa stain is not required for real-time PCR b. It allows for species identification and quantification of malaria-causing plasmodium at lower blood concentration with greater sensitivity and specificity. c. Real-time PCR doesn't require the use of a microscope which is deemed too expensive. d. Real-time PCR has a hiher sensitivity for the main malaria-causing plasmodium, P. falciparum, than microscopy techniques.

What are the advantages of real-time PCR over microscopy for diagnosing malaria? a. Giemsa stain is not required for real-time PCR b. It allows for species identification and quantification of malaria-causing plasmodium at lower blood concentration with greater sensitivity and specificity. c. Real-time PCR doesn't require the use of a microscope which is deemed too expensive. d. Real-time PCR has a hiher sensitivity for the main malaria-causing plasmodium, P. falciparum, than microscopy techniques.

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter15: Instrumentation In Medical Imaging

Section: Chapter Questions

Problem 6Q

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When using a micro centrifuge, what should you check before starting the machine? A. That the samples are balanced B. That the PCR tube caps on the samples are slightly open C. That the machines is set to 64 degrees Celsius D. That is programed for 35 cycles.
Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.
A) What are the three main steps involved in PCR? Include temperatures and descriptions of each step. B) Explain why RFLP can produce many bands on an electrophoresis gel and PCR (one set of primers) will only produce one or two bands on a gel for the same genome.
Increasing the tube voltage on a clinical x-ray unit: a. Improves the resolution of an x-ray image. b. Increases the intensity of the Characteristic x-rays. c. Increases the intensity of the Bremsstrahlung x-rays. d. Increases the energy of the Bremsstrahlung x-rays. e. Increases the energy of the Characteristic x-rays. and why??
Answer each of the following questions correctly. a. How PCR may be used for the detection of disease–causing pathogens in a population during COVID Pandemic. For example: It may be used to check if a patient has a COVID virus infection. b. How cloning and expression of certain genes allows for massive production of the desired product. For example: the cloning and expression of insulin in bacteria allows for the mass production of this necessary protein for use by diabetic patients.
How could you determine if the white stringy material was DNA? A. By using spectrophotometer B. By adding ice cold ethanol C. By high speed centrifugation and observation of the pellet D. By deproteinization E. By looking at the unstained strings under the microscope
a. What determines the size (length) of the primary PCR product? b. What might a successful gel check of a PCR reaction look like?
If the PCR analysis of your sample did not work can you suggest why? How can you test if this is true?
a. Explain whether or not DNA polymerase from a mesophilic bacterium could be used successfully in a PCR reaction. b. If starting with a single double-stranded DNA molecule, how many copies of the DNA would be synthesized after 25 PCR cycles?
In what way could the extreme sensitivity of the PCR method be aproblem when working with clinical specimens?
Explain briefly within 5 sentences each 1. a. What is PCR? Explain/relate its importance in DNA marker analysis. 1.b. What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.
Explain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.