Why there is no detectable fluorescence in the samples after the first few PCR cycles? O A. Because there is no DNA synthesis. O B. Because the quenchers block the fluorescence of all reporter dye molecules. O . Because Taq polymerase degrades the TaqMan probe molecules. D. Because the fluorescence detector is not sensitive enough. E. Because Taq polymerase degrades the primers.

Why there is no detectable fluorescence in the samples after the first few PCR cycles? O A. Because there is no DNA synthesis. O B. Because the quenchers block the fluorescence of all reporter dye molecules. O . Because Taq polymerase degrades the TaqMan probe molecules. D. Because the fluorescence detector is not sensitive enough. E. Because Taq polymerase degrades the primers.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 10QP: Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands...

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A) What are the three main steps involved in PCR? Include temperatures and descriptions of each step. B) Explain why RFLP can produce many bands on an electrophoresis gel and PCR (one set of primers) will only produce one or two bands on a gel for the same genome.
a. Explain why the PCR is unlikely to amplify contaminatingbacterial DNA in a sample of human DNA. b. Explain how PCR could be used to pick a gene out of a complexgenome and amplify it.
Explain why the PCR is unlikely to amplify contaminating bacterial DNA in a sample of human DNA.Explain how PCR could be used to pick a gene out of a complex genome and amplify it.
What is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' direction
(A) After three cycles of PCR, how many DNA molecules are present that correspond precisely to the desired amplification product? (B) What about after 5 cycles. Assume that we started with one molecule in each case, and that the reaction is perfectly efficient.
Which process consists of modifying an individual’s genome? a. DNA profiling b. SNPing c. genetic engineering d. DNA hybridization e. PCR
Quantitative PCR (qPCR) has one extra ingredient to quantify DNA. Describe its structure and explain how it changes during the reaction.
a. Describe the ingredients of a PCR reaction. b. List the different temperatures used in each cycle and tell why each is used.
Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.
Which technique rapidly replicates specific DNA fragments without cloning in cells? (a) gel electrophoresis (b) cDNA libraries (c) DNA probe (d) restriction fragment length polymorphism (e) polymerase chain reaction
Would it be possible to use human polymerase for the PCR reaction? a. No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur. b. No, because human polymerase cannot be extracted from cells to use in a lab setting. c. Yes, because we are using human DNA as the template DNA. d. Yes, because human polymerase can add bases to a template strand without a primer.
a) what are restriction enzymes? b) What is the main function of restriction enzymes in nature? c) Compare and contrast the these enzymes in nature and in scientific research.