What colony characteristics can be used for differentiation of bacterial species? As an example, compare the properties of colonies of Serratia marcescens and Micrococcus luteus on your streak plate.
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What colony characteristics can be used for differentiation of bacterial species? As an example, compare
the properties of colonies of Serratia marcescens and Micrococcus luteus on your streak plate.
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- What is the importance of describing the morphological characteristics of bacterial colonies when cultures were sampled from an environmental source? Explain and justify your points.The smear prepared from the gram-positive control shown gram-negative results. Describe 3 reasons why this incorrect reaction could occur Can the possession of flagella by bacteria be utilized for classification purposes? Explain.what would be the medical significance of finding a positive endospore stain result from bacilli bacteria obtained from a sample of honey that was fed to a baby wtih severe gastrointestinal distress?
- Why is it important to follow sterile technique? What can happen to cause gram-positive bacteria to appear gram-negative?When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. The alcohol wash removes crystal violet and iodine from the bacteria. Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.How would the slide appear if the bacteria were stained with safranin, a basic red dye?
- Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria responsible in any way for the Gram stain reaction?Can the presence and absence of capsules in bacteria be used to identify unknown bacteria? YES OR NO? WHY?How can colony morphology be used in the identification of bacterial species? What are the environmental factors that affect the growth of bacteria? What is agar (or agar-agar)? Is this different from nutrient agar? Why? How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose? For what purpose would you adjust each of the following microscope components during a microscopy exercise? iris diaphragm coarse adjustment knob fine adjustment knob condenser mechanical stage control
- What technique is used to stain this bacterium? What kind of stain is used? What type of chromophores does this stain use? What is the purpose of this technique? Do we heat fix when performing this technique?You prepared another slide to visualize the bacteria under the oil immersion objective lens, but you found that the oil present in the lab was labeled as “vegetable oil”. Do you think it will be possible to use this oil to observe bacteria clearly? Explain your answerWhat is the difference between gram positive and gram negative bacteria? Describe how we use this to our advantage in the gram staining process. Hint: Gram positive versus Gram negative!!!