What are the disadvantages of using the mouth in filling a pipette? Why do we use exponential notation in counting bacteria? Why is serial dilution necessary?
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- What are the disadvantages of using the mouth in filling a pipette?
- Why do we use exponential notation in counting bacteria?
- Why is serial dilution necessary?
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- Why is E. coli O157:H7 an organism of concern in contaminated foods? The strain is represented as O157:H7. Which particular “parts” of the bacterial cell do the “O” and “H” refer to?1 mL of a juice sample is taken and diluted in 9 mL culture medium, subsequently, this suspension is analyzed in the Petroff-Hausser chamber, determines that the total number of bacteria found is 44 bacteria per square, if the chamber has 25 squares, how many bacteria are in the chamber? initial juice sample?How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume? suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10^-1 dilution of the entire culture. Explain how you would do this. Show your calculations.
- Given a log phase bacterial culture with 1 x 10^6 cells per ml and a generation time of 30 minutes how long does it take the culture to reach a density of 6.4 x 10^7 cells per ml?What are the defining characteristics of the exponential/log phase of the bacterial growth curve. Explain the culture conditions that arise that cause the phase to end. In different culture media, and the exact same starting inoculum, will the end of exponential/log occur at the same time? Why or why not? A graph or a drawing of the growth curve would help but is not essential to answer this question.From a soup sample, 1 mL of sample is taken and diluted in 99 mL of soup medium. culture, later, this suspension is analyzed in the Petroff-Hausser chamber, It is determined that the total number of bacteria found is 53 bacteria per square, if the chamber has 25 squares, how many bacteria are found in the chamber? initial juice sample?
- The images attached are the photos of bacteria in yoghurt under a misroscope. According to these images and your own knowledge, can you make a biological drawing of bacteria in yoghurt ( in a circle) and identify the types of bacteria accurately?What is G+C content of a bacteria? How it can be determined? Why it is not safe to assume that microorganisms with same G+C content belong to same species? How G+C content data is taxonomically valuable?In this activity, you will receive an “unknown” containing two species. The first step in identifying these bacteria is to __________. In this activity, you will receive an “unknown” containing two species. The first step in identifying these bacteria is to __________. inoculate a glucose fermentation tube perform an acid-fast stain streak a TSA plate for isolation inoculate two TSA slants inoculate two OF-glucose tubes
- Why do acid-fast positive bacteria grow more slowly?What technique is used to stain this bacterium? What kind of stain is used? What type of chromophores does this stain use? What is the purpose of this technique? Do we heat fix when performing this technique?What technique is used to stain this bacterium? What kind of stain is used? What type of chromophores does this stain use? What is the purpose of this technique? What is a smear? Do we heat fix when performing this technique?