What does it mean if the closest matched organism that is returned from a BLAST search is less than a 97% match to an organism you are sequencing
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What does it mean if the closest matched organism that is returned from a BLAST search is less than a 97% match to an organism you are sequencing?
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- What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis profile from the Sanger sequencing method?How does what you see on the acrylamide gel reflect what is presented on a sequencing chromatograph?How do you interpret Blastn results with 0 % query coverage and 100 % identity?
- Given the electrophoresis profile of a Sanger sequencing result, what was the sequence of the original DNA sample used for sequencing? GGTAACC CCAATGG GGTTACC CCATTGGHow does the shotgun approach differ from the whole genome sequencing?What would be the final primer concentration if 0.5 μl of 10 μM primers were added to a PCR reaction with a final volume of 20 μl?