What is the relationship between fluorescence intensity of a spot and the amount of DNA in a sample for a Virochip DNA microassay? From the following which is the best choice Less DNA results in greater fluorescene since the laser passes through he sample eaiser More DNA equals to more intense fluorescence since the DNA makes a layer to refelct the laser light More DNA equals to more intense fluorescence since of more dye on the DNA is fluorescene More DNA results in to more fluorescence since there's more DNA to be excted by the laser None of the above

What is the relationship between fluorescence intensity of a spot and the amount of DNA in a sample for a Virochip DNA microassay? From the following which is the best choice Less DNA results in greater fluorescene since the laser passes through he sample eaiser More DNA equals to more intense fluorescence since the DNA makes a layer to refelct the laser light More DNA equals to more intense fluorescence since of more dye on the DNA is fluorescene More DNA results in to more fluorescence since there's more DNA to be excted by the laser None of the above

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...

See similar textbooks

Similar questions

Which of the following best describes the process of DNA seqencing. a. DNA is seperated on a gel and the different bands are labled with flouroscent nucleotides and scanned with a laser. b. A laser is used to flurorescently label the nucleotides present with in the DNA , the DNA is run on a gel and then the DNA is droken into fragments c. Nucleotides are scanned with a laser and incrprorated into the DNA that has been seperated on a gel and then DNA is amplified with PCR. d. fragments of DNA are produced in a reaction that lables them with any of four different fluroscent dyes and the fragmented then are run on a gel and scanned with laser e. DNA is broken down into its constituents nucleotides and the nucleotides are then run on a gel and purified with a laser
Samples came to the laboratory for analysis. You are asked to identify the differences between these examples. It is known that the DNA of the samples must first be isolated in order to understand the difference. Isolated to DNA. It's time for the next step. It was decided to use the RAPD technique. The amount of DNA in the samples isolated in the spectrophotometer was determined. The sample is measured at 250 ng / microliter. In the RAPD technique, the total reaction volume for PCR is optimized to be 15 microliters. This is adjusted to contain 5 microliters of DNA in 15 microliters. This technique requires 25 ng of DNA to work. How to dilute the measured 250 ng / microliter sample DNA into the appropriate amount for the reaction to occur?
Three pieces of cut DNA were isolated using gel electrophoresis.The DNA that is the longest will be closest to the positive or negative electrode?
Which of the following is not true about microarrays? Group of answer choices A microarray is a glass slide with single-stranded DNA attached to it. A microarray is covered in spots, where each spot represents a different sequence that will be assessed in the experiment. A microarray can assess hundreds of thousands of variants simultaneously. The same microarray slide can be used either for DNA microarrays or RNA microarrays
What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with the amount of PCRproduct.
You are given the following DNA fragment to sequence:5'-GCTTAGCATC-3'. You first clone the fragment in bacterialcells to produce sufficient DNA for sequencing. You isolate theDNA from the bacterial cells and carry out the dideoxy-sequencingmethod. You then separate the products of the polymerizationreactions by gel electrophoresis. Draw the bands that shouldappear on the gel from the four sequencing reactions.
During DNA profiling, DNA nucleotides hybridized with probe can be detected througha) Electrophoresisb) Polymerase chain reactionc) Autoradiographyd) Hybridoma
Which of the following statements is true of a Sanger sequencing reaction but not colony PCR? Select all that apply. Must contain only a single primer ddNTPs are added Uses a heat stable DNA polymerase Requires a pair of primers that bind to opposite strands Requires temperature cycling
A student wanted to load .75 ug dna on agarose gel and has 4x loading buffer for sample preparation. The student has 50ul purified plasmid, finding concentration of plasmid to be 250 ng/uL The student used 10uL plasmid DNA in 50uL reaction for the restriction digest Give the volumes of restriction digest and 4x loading buffer that student would mix together and load on agarose gel.
DNA fingerprinting uses a process called gel electrophoresis to separate the fragments of DNA. Once the DNA fragments are sorted, the pattern of bands can be analyzed. Gel Electrophoresis Procedure The smaller DNA fragments start to move away from the wells and the larger DNA fragments remain closer to the wells. An electric current is passed through the gel. DNA fragments are treated with a dye. A restriction endonuclease is added to the DNA. Using micropipettes, the DNA samples are added to the wells. DNA fingerprint is produced. DNA fragments are produced. The order in which a DNA fingerprint is produced using gel electrophoresis is Answer, Answer, Answer, Answer, Answer, Answer, and Answer.
What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with the level of PCRproduct.
When gel electrophoresis is done correctly, which of these DNA molecules would move the least through the gel? Group of answer choices: 1000 bp molecules 6 Kbp molecules 3 Kbp molecules 2000 bp molecules