What hypothesis/assumptions can be derived from the determination of reducing sugars using the DNS (dinitrosalicylic acid) method?
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What hypothesis/assumptions can be derived from the determination of reducing sugars using the DNS (dinitrosalicylic acid) method?
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- Give a short explanation of the process of the DNS (dinitrosalicylic acid) assay/method in determining and examining reducing sugars.how are SDS-PAGE and BN-PAGE techniques used determine the composition of protein-photosynthetic complexes? also how do they work? please be thorough in the explanation!What method and conditions (provide detail) do you propose to measure the Specific Activity of the enzyme chymotrypsin?
- Why is ΔG a useful quantity for predicting the favorability of biochemical reactions?Briefly explain the mode of action of taxanes in relation to the experiment we carried out. What uses might taxanes have as a therapeutic drug?Why is a Lineweaver–Burk plot useful in analyzing kinetic data from enzymatic reactions?
- Please asnwer the following question: Suppose you specifically want to measure the amount of beta-glucose present in a sample. You have an enzyme that reacts specifically with the beta form of the sugar,and produces a signal that is easy to measure. However, every time you try to measure the amount of beta-glucose, mutarotation occurs and the alpha form of the sugar is converted into beta!! Can you suggest a simple way of overcoming this problem so you could measure only the beta form of the sugar? HINT: Is there a way you can prevent mutarotation?Draw in detail the pathway of de novo synthesis of deoxythymidine phosphate and provide a brief explanation.Which biochemical reactions are taking place on a molecular level during cocoa bean fermentation?
- What are revision products? What type of reaction is it? What is the common intermediate reversion product of glucose and fructose? What conditions favour the reaction?Briefly explain how ester hydrolysis experiments helped to provide evidence for the mechanism of peptide hydrolysis catalyzed by chymotrypsin.Briefly comment on the differences of using a fixed-time assay versus a kinetic assay to measure enzyme activity. Is it reasonable to assume that the reaction velocity obtained by measuring the amount of product after 30 minutes in a fixed-time assay is directly proportional to absorbance? How could you determine whether this was the case? Word limit 180 words including citation and reference