Two-dimensional gel electrophoresis of proteins in a cell extract provides a qualitative way to compare proteins with respect to intra- cellular abundance. Describe a quantitative approach to the deter- mination of number of molecules of an enzyme per cell.
Q: Why is ΔG a useful quantity for predicting the favorability of biochemical reactions?
A: Gibbs free energy change (ΔG) is the most useful measure for predicting the direction of chemical…
Q: Two-dimensional gel electrophoresis of proteins in a cell extract provides a qualitative way to…
A: Two-dimensional electrophoresis is a form of gel electrophoresis used to analyze proteins. Mixtures…
Q: Summarize the data below obtained from the protein experiments for determination of protein…
A: The principle of the Bradford assay is based on the fact that the absorbance maximum for an acidic…
Q: xplain what is meant by Optimum pH. Does pH lower than optimum pH denature the enzyme? How about pH…
A: Enzymes are biocatalysts that fasten the rate of chemical reactions. It decreases the activation…
Q: You have performed protein purification on your new favorite enzyme using a protocol which involves…
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A: Suicide inhibition, also known as suicide inactivation is a form of irreversible form of enzyme…
Q: H3. Draw the Lineweaver-Burk plots for the reaction that your selected enzyme is involved with and…
A: Inhibitors are substances which inhibit the activity of an enzyme. Inhibitors bind to the active…
Q: In the course of purifying an enzyme, a researcher performs a purification step that results in an…
A: An enzyme is a biocatlyst that increases the rate of overall reaction. Activity of enzyme is…
Q: Compare the KM and Vmax of the enzyme in the absence and presence of inhibitors to determine what…
A: Inhibitors are the molecules which decrease the efficiency of enzyme activity.
Q: Using the data in the table below, calculate the average molar mass of an amino acid residue in each…
A: On the basis of composition, proteins are classified as simple or conjugated. Simple proteins, such…
Q: giving an overview of the use of colorimetric enzyme assays, as well as discussing their use in…
A: Colorimetric assays are reactions that lead to a change of color due to an enzymatic or chemical…
Q: Mention assays other than biuret and lowry assays that are used to detect the presence of proteins.…
A: Introduction: Protein is the major macromolecule that plays a vital role in the structure and…
Q: Describe a general scheme for protein purification of a protein like hemoglobin
A: Protein Purification: The protein Purification is done is to retain more number of function proteins…
Q: Why is the regulation of biochemical processes important?List three reasons
A: The reactions which take place in living beings are called biochemical processes. The regulation of…
Q: During successufl purification of every enzyme, the following may be expected: 1. Solubility in NaCl…
A: The specificity increases during the purification of every enzyme.
Q: What is the qaulitative abstract for Color Reactions of Proteins and Amino Acids? (one paragraph…
A: One paragraph abstract on the topic: Colour reactions of proteins and amino acids outlining…
Q: Which of the following analogies best describes the induced-fit model of enzyme-substrate binding?…
A: Each enzyme consists of a substrate binding site which is known as active site. When a substrate…
Q: Essay: In your own words explain the following concept in not more than 5 sentences a. The lock and…
A: A. The lock and key model showing enzyme-substrate interaction. In which the substrate behave like…
Q: Why is a Lineweaver–Burk plot useful in analyzing kinetic data from enzymatic reactions?
A: Gerenally, The Lineweaver–Burk plot was wide accustomed to confirm vital terms in protein kinetics,…
Q: In the Biuret Assay for protein concentration determination, the role of sodium potassium tartrate…
A: The biuret test is a chemical test that can be performed to determine whether an analyte has peptide…
Q: Why different amino acids have different Rf values? If you separate a mixture of amino acids consist…
A: Paper chromatography is the separation technique where the mixture of the amino acids was separated…
Q: Enzymes are stereochemically specific; that is, they oftenconvert only one stereoisomeric form of…
A: enzymes are proteins that are made up of amino acids. Enzymes contain active sites. These active…
Q: During successful purification of every enzyme, the following may be expected: Select ALL that…
A: Enzyme purification and characterization is of great importance in obtaining information about…
Q: Only a few amino acid residues are actually involved in catalysis in enzymes, yet enzymes are…
A: Functions for non-catalytic amino acids: Scaffolding of signaling components: these are those…
Q: If you did not use a saturating concentration of pNPP to establish your initial calibration curve,…
A: p-Nitrophenyl Phosphate (PNPP): It is a non-proteinaceous, non-specific substrate for protein,…
Q: Explain and differentiate the lock-and-key and induced fit models for binding of a substrate to an…
A: Enzymes are biocatalysts that speed up biological reactions. Enzymes interact with substrates and…
Q: Provide examples of biochemical phenomena that are directly affected by macromolecular crowding.
A: Macromolecular crowding refers to the the accumulation of large molecules such as protein, in the…
Q: give the significance/role/effect of the reagent/condition in the isolation or analysis of a…
A: The classical method of lipid extraction from egg yolk is the 2-Propanol/Hexane solvent extraction…
Q: topic: Determination of Protein Concentration by Spectrophotometry Enumerate and discuss the…
A: Spectroscopy is the interaction between electromagnetic radiation and matter. It works on the…
Q: Using linear regression analysis, determine the values of V, and KM of the enzyme in the PRESENCE of…
A: The enzyme binds to the substrate form an enzyme-substrate complex in an enzyme-catalyzed reaction.…
Q: Discuss the advantages and disadvantages of Protein efficiency ratio(PER)
A: Protein efficiency ratio (PER) is the weight gained by a test subject divided by its intake of a…
Q: With a branched biochemical pathway, why would it be important for a cell to shut down the first…
A: Metabolism refers to all biochemical reactions that occur in a cell. It is classified into 2 types…
Q: how are SDS-PAGE and BN-PAGE techniques used determine the composition of protein-photosynthetic…
A: PAGE OR the polyacrylamide gel electrophoresis is a kind of molecular technique that involves the…
Q: DNS reaction is an alternative assay for enzyme activity .What is involved or basis for this…
A: DNS or Dinitrosalicylic acid reaction is used to detect the presence of reducing sugars present in…
Q: Parallel lines on a Lineweaver-Burk plot are diagnostic of— noncompetitive inhibition.…
A: The answer is option e The answer is uncompetitive inhibition
Q: A plot of 1/ versux 1151, called a Lineweaver Burk or double-reciprocal plol, is a useful tool for…
A: Enzymes are proteins that hasten biochemical reactions such that substrate molecules are converted…
Q: Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the ab- sence…
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…
Q: The turnover number for an enzyme is known to be 5000min-1. Given the following set of data,…
A: Enzyme kinetics is defined as the study of chemical reactions which are catalyzed by enzymes. Km is…
Q: (a) From the information given in the table, calculate the specific activity of the enzyme, total…
A: Specific activity of enzymes is defined as the activity of the enzyme per milligram of the total…
Q: 200 ml of a 2% protein solution are available, containing an enzyme to be purified. Half of the…
A: Protein is a macromolecule made up of monomers aminoacids. Protein helps maintain the structural…
Q: _____________ such as dextran are used to simulate intracellular conditions in enzyme studies
A: Introduction: Enzymes are proteins in nature that act as biological catalysts and increase the rate…
Q: List down the properties of an ideal carrier matrix for enzyme immobilization.
A: Enzymes can be used as catalytic agents in medical and industrial applications. Such enzymes are…
Q: Using linear regression analysis, determine the values of Vmax and KM of the enzyme in the PRESENCE…
A: Michaelis menten constant, Km is the substrate concentration required to produce half maximum…
Q: • What happens to the functionality of a denatured enzyme? • How can that result be explained with…
A: Enzymes are biological machines that may catalyze certain processes in the cell. Enzymes catalyze…
Q: Why is it useful to plot rate data for enzymatic reactions as a straight line rather than as a…
A: Enzymes are the biocatalyst that increases the rate of biochemical reactions. where its ability…
Q: Is the data that you are collecting in the above table quantitative or qualitative? Explain why.…
A: Background Information Enzyme activity: It is defined as the rate at which reactants are converted…
Q: If 10 µM enzyme was used to obtain the data in the summary plot below, k-cat = ________________…
A: An enzyme is a type of biological catalyst that aids in the acceleration of chemical reactions.…
Q: A two-dimensional gel electrophoresis of a protein preparation revealed the presence of five…
A: Size exclusion chromatography is another name for gel filtration chromatography. Gel filtration…
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- Is the data that you are collecting in the above table quantitative or qualitative? Explain why. Which treatment had the least amount of browning? Which had the most? Why do you think you obtained these results? Remember that the enzyme polyphenol oxidase is a protein! For each treatment, apply your knowledge of how temperature, pH, and salt concentration affect enzyme activity and explain why you got the results that you did. Include bonds and the levels of protein structure that you explored in Activity A in your answer. How does temperature impact the rate of enzyme activity? If you were to leave the apple in the refrigerator longer, why would it eventually brown? Explain based on what you know about enzyme activity. How does pH and its impact on specific types of bonds explain the results you obtained in your lemon juice treatment? Include bonds and levels of protein structure in your answer. How does salt and its impact on specific types of bonds explain the results you obtained…During successufl purification of every enzyme, the following may be expected: 1. Solubility in NaCl increases 2. The activity increases 3. The specificity increases 4. The number of subunits increases 5. the epitope number increasesMechanistic probes often require the use of an electrophilic functional group to interact with an especially-reactive amino acid in the protein active site. For each of the follow functional groups that can be used in a mechanistic probe, provide a structure of the product. asap
- Please answer clearly and directly Explain what is meant by Optimum pH. Does pH lower than optimum pH denature the enzyme? How about pH higher than optimum value?Why is ΔG a useful quantity for predicting the favorability of biochemical reactions?topic: Determination of Protein Concentration by Spectrophotometry Enumerate and discuss the limitations of Beer-Lambert’s Law.
- Briefly comment on the differences of using a fixed-time assay versus a kinetic assay to measure enzyme activity. Is it reasonable to assume that the reaction velocity obtained by measuring the amount of product after 30 minutes in a fixed-time assay is directly proportional to absorbance? How could you determine whether this was the case? Word limit 180 words including citation and referencegiving an overview of the use of colorimetric enzyme assays, as well as discussing their use in deriving the Vmax and Km: two parameters that define the kinetic behaviour of an enzyme as a function of the substrate concentration [S].True or False Immobilization improves the stability of the enzyme. EnaLne, has a half-life of 10 days in free solution, but under identical conditions of temperature, pH, and medium composition, the measured half-life of a packed column is 30 days. The enzyme is immobilized in a porous sphere 5 mm in diameter.
- SPR is one of many techniques to examine binding affinities of biomolecules. Choose another technique that can be used to measure binding affinity between two biomolecules, and briefly compare and contrast this technique with SPR. (Hint: think about sample requirements, the type of data produced, potential pitfalls with the techniques, etc.)FRET is a widely used biophysical technique for the characterization of a wide range of biomolecular interactions. Give a brief description of how a FRET experiement is done.DNS reaction is an alternative assay for enzyme activity .What is involved or basis for this reaction?