What is a reporter gene? The product of which reporter geneyields a green color?

Q: What is the general effect of methylation of a genomics region on transcription?

A: DNA methylation is a biochemical process involving the addition of methyl groups to the Dna strand.…

Q: How do you clone a 4 kb promoter from a genomic cell preparation?

A: Genome is the complete set of genetic material present in the nucleus of a cell. It houses all the…

Q: What is a simple way of making a knockout mouse in terms of DNA recombinant technology?

A: A knockout mouse, or knock-out mouse, is a genetically modified mouse in which researchers have…

Q: What are strong promoters?

A: The strength of a promoter is the rate of transcription of the gene controlled by this promoter.

Q: Describe the function of a reporter gene.

A: Gene is the primary fundamental unit. These are made up of Deoxyribonucleic acid (DNA). These are a…

Q: Does use of nucleic acid probes depend on gene expression?

A: Introduction: Nucleic acid hybridization or nucleic acid probe are used to determine specific gene…

Q: How reliable does the rbcL gene work as a barcode for identifying plant species?

A: * DNA barcoding is method to identify  a species by using a short section of DNA. They will compare…

Q: What are the application of rice genome?

A: Introduction  Domestic Rice (Oryza sativa) is the most important cereal crops. It is the staple food…

Q: What are the basic steps involved in cloning foreign DNA into a vector?  How do you differentiate an…

A: Cloning Cloning refers to the making of identical copies. DNA cloning is a molecular biology…

Q: What are the components needed for the processes of transformation conjugation and transduction? How…

A: The recipient bacterium during transformation occurs in the extracellular donor DNA. The donor DNA…

Q: What is the job of the restriction enzyme?

A: In the year 1963 the two enzymes responsible for restricting the growth of bacteriophage in E. coli…

Q: What are transgenic plants?Give some example.

A: The genetically modified organism’s also known as bioengineered foods. There are several steps in…

Q: What is a chimera and how is it different from a “true transgenic animal”?

A: A chimera is an single organism with DNA of two or three different types in it. For example during…

Q: What is a Paralog and How do you identify a paralogous gene?

A: A gene is a unique sequence of nucleotides which forms a part of a chromosome, which determines the…

Q: How is gene Z used as a marker?

A: β-galactosidase, also known as lactase, beta-gal or β-gal, is a glycoside hydrolase  enzyme which…

Q: In gene mapping using generalized transduction, bacterial genes that are cotransduced are a. far…

A: Transduction is a process used to map the bacterial genes. The transduction is of two types,…

Q: Compare and contrast the use of histochemical reporter genes and fluorescent reporter genes — what…

A: Reporter genes can be analyzed by different methods, such as beta glucuronidase (uidA) gene…

Q: What are proximal promoter elements ?

A: Gene is the structural and functional unit in the DNA. Genes are composed of nucleotide nitrogenous…

Q: Can the Cloned Genes in LibrariesBe Expressed?

A: DNA libraries are collection of cloned genes. These cloned genes are such large that they can…

Q: What is double-knockout animals (DKOs) ?

A: Gene is the basic unit of heredity of an organism. In most animals, Deoxyribonucleic acid (DNA) is…

Q: hat is subcloning

A: Biology is a branch of science. Bio means life and ology means study. Biology is basically the study…

Q: Why is the technique used to clone mammals called somatic cellnuclear transfer?

A: A somatic cell is a cell that takes part in the composition of the body of an organism and divides…

Q: What is gene tagging?

A: Introduction Gene functions as the key to control almost all the physiochemical, biochemical…

Q: Vhat is the main difference between eukaryotic and prokaryotic gene regulation?

A: As prokaryotic organism lack nucleus, DNA (Deoxyribonucleic acid) floats freely in this single…

Q: What is the utility of a reporter gene?

A: Introduction Reporter gene are the genes which helps in detection of transformant cell as their…

Q: How can the number of repeats of a microsatellite/STR increase?

A: A repetitive DNA tract wherein a particular motif of DNA is repeated 5 to 50 times is termed a…

Q: Explain how a reporter sequence can be used to provide information about the expression pattern of a…

A: In the field of genetic engineering, the gene of interest is attached to a specific gene called the…

Q: What is gene gun ?

A: The technique of tissue culture is used for genetic engineering. In this method tissue samples are…

Q: What is the Use of a promoterless lacZ gene?

A: In prokaryotes, the DNA-binding protein controls the expression and function of genes by controlling…

Q: What are the functions of primer and Taq polymerase?

A: Polymerase chain reaction used to make thousands of copies of DNA from very small amount of the DNA…

Q: Explain what are dispersed core promoters ?

A: Transcription is the process of transferring information from DNA to a single-stranded molecule…

Q: What are reporter gene ?

A:

Q: What is CRISPR?

A: Introduction  Bacteria and viruses are tough competitor to each other, in order to overcome each…

Q: To make Dolly the clone, they first isolated a cell from where?

A: Dolly ,a sheep created or made by the fusion of mammary gland nucleus to the enucleated egg cell of…

Q: What are CRISPR-associated (cas) genes ?

A:

Q: For a translational reporter, is GUS/lacZ the best option, or would other reporter genes provide…

A: Ans  - The LacZ gene from E. coli is frequently used as a reporter gene because it produces a blue…

Q: What are pseudogenes and how are they produced? Are pseudogenes expressed?

A: The DNA is the genetic material that is passed from one generation to the next generation. It is…

Q: What are dispersed core promoters  ?

A: Transcription is the process by which the information contained in the DNA is copied to a molecule…

Q: What is CRISPR? What can it do?

A: Unlike eukaryotic cells in plants and animals, prokaryotic cells such as bacteria and archaea have…

Q: Explain how to determine whether a given clone contains all or only part of the gene ?

A: Gene cloning is described as the process that includes a relocation of gene of interest and which is…

Q: What is the key property of a reporter gene?

A: Reporter genes are used in molecular biology. They are referred to those nucleic acid sequences that…

Q: What are transgenic plants?

A: Transgenic means to add one more DNA sequences from another species by artificial means. Mostly, In…

Q: How do you design a reporter assay for Serpina1?

A: The gene has two parts. The first is the coding sequence which gets transcribed to RNA and…

Q: What is   transgenesis?

A: A gene is a sequence of nucleotides in RNA or DNA that encodes the synthesis of a gene product,…

Q: What are Focused core promoters ?

A: Promoters are DNA regions where gene transcription begins. Focused core promoters are one of the two…

Q: How does a promoter dictate which DNA strand is used as the template?

A: The promoter region is referred to as the site where the process of transcription begins on the DNA…

Q: How is CRISPR-Cas used to introduce specific changes into DNA sequences?

A: Bacteria’s are the prokaryotic microbial cells which have incipient nucleus. Most of the bacteria’s…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.