What is the key property of a reporter gene?

Q: What is the general effect of methylation of a genomics region on transcription?

A: DNA methylation is a biochemical process involving the addition of methyl groups to the Dna strand.…

Q: How do you clone a 4 kb promoter from a genomic cell preparation?

A: Genome is the complete set of genetic material present in the nucleus of a cell. It houses all the…

Q: What is a forward genetic screen?

A: Genetics is a division of biology that includes study of heredity, genes and genetic variation.…

Q: What are strong promoters?

A: The strength of a promoter is the rate of transcription of the gene controlled by this promoter.

Q: What is the initial product of the transcription process?

A: Introduction: The process by which DNA molecule form a RNA molecule that is messenger RNA (mRNA) is…

Q: Describe the function of a reporter gene.

A: Gene is the primary fundamental unit. These are made up of Deoxyribonucleic acid (DNA). These are a…

Q: How does a host cell harboring a restriction enzyme protect its own DNA?

A: host cell harboring a restriction enzyme protect its own DNA by :

Q: What are the basic steps involved in cloning foreign DNA into a vector?  How do you differentiate an…

A: Cloning Cloning refers to the making of identical copies. DNA cloning is a molecular biology…

Q: What are the components needed for the processes of transformation conjugation and transduction? How…

A: The recipient bacterium during transformation occurs in the extracellular donor DNA. The donor DNA…

Q: How geneticists used synthetic mRNAs to limit the coding possibilities?

A: Prior to the technology existed for RNA synthesis of defined sequence such as given in the figure…

Q: What is a Paralog and How do you identify a paralogous gene?

A: A gene is a unique sequence of nucleotides which forms a part of a chromosome, which determines the…

Q: How is gene Z used as a marker?

A: β-galactosidase, also known as lactase, beta-gal or β-gal, is a glycoside hydrolase  enzyme which…

Q: What are selectable markers and why are they important to cloning vectors?

A: In recombinant DNA technology the vectors are the carrier for the target DNA sequence which needed…

Q: What is a multiple cloning site, and what is insertionalinactivation?

A: A DNA segment characterized by the presence of multiple restriction sites is known as the multiple…

Q: What are proximal promoter elements ?

A: Gene is the structural and functional unit in the DNA. Genes are composed of nucleotide nitrogenous…

Q: What are some of the challenges of expressing a eukaryotic gene in a prokaryotic host cell?

A: In the process of genetic engineering, the gene of interest can be used to produce several gene…

Q: What is the unit of a genetic map?

A: Genetic map:   Gene mapping as the name suggests that it is the map of genes that are present inside…

Q: Why are nucleic acid probes necessary to lyse cells containing plasmids in order to detect the…

A: Cell lysis is frequently the very first step in most purification techniques for nucleic acids,…

Q: What is gene tagging?

A: Introduction Gene functions as the key to control almost all the physiochemical, biochemical…

Q: What role do CRISPR-Cas systems naturally play in bacteria?

A: Bacteria are single-celled microscopic prokaryotes. They are organized into five groups based on…

Q: How long is the CFTR gene and what are the first 20 nucleotides of this gene

A: CFTR Gene- CFTR is a Cystic fibrosis transmembrane conductance regulator. It is a membrane protein…

Q: What is a restriction fragment?

A: A restriction fragment can be defined as the amount of  DNA fragment which occurs due to the cutting…

Q: What is the role of a vector in gene therapy?

A: Gene therapy is based on the premise that replacement of a disease-causing gene with the healthy…

Q: What is the utility of a reporter gene?

A: Introduction Reporter gene are the genes which helps in detection of transformant cell as their…

Q: What is the role of the following molecules in the CRISPR mechanism? *

A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These are a set of DNA…

Q: How can the number of repeats of a microsatellite/STR increase?

A: A repetitive DNA tract wherein a particular motif of DNA is repeated 5 to 50 times is termed a…

Q: What does the ABL gene do?

A: The deoxyribonucleic acid (DNA) is the hereditary material that transmits the genetic information…

Q: What is the function of a restriction enzyme?

A: Restriction enzymes are enzymes that are isolated from bacteria that can cleave single-stranded or…

Q: What is gene gun ?

A: The technique of tissue culture is used for genetic engineering. In this method tissue samples are…

Q: What are the functions of primer and Taq polymerase?

A: Polymerase chain reaction used to make thousands of copies of DNA from very small amount of the DNA…

Q: Where did western blot get its name?

A: The western blot (also known as the protein immunoblot) or western blotting is molecular biology and…

Q: What are reporter gene ?

A:

Q: What is the basic process of Gene therapy?

A: Gene therapy is the altering the genes or effects of genes inside patient's body to prevent or cure…

Q: What are conditional knockout mice?

A: A knockout mouse (conventional knockout) is a genetically modified mouse in which the researchers…

Q: what is the purpose of the neo+ gene that is attached to the Bt gene?

A: Gene is the basic functional and physical unit of heredity. They are made up of DNA and are…

Q: For a translational reporter, is GUS/lacZ the best option, or would other reporter genes provide…

A: Ans  - The LacZ gene from E. coli is frequently used as a reporter gene because it produces a blue…

Q: What is the purpose of transgenesis?

A: Genetic engineering is the process of using recombinant DNA (rDNA) technology to alter the genetic…

Q: What is a common proximal-promoter element ?

A: Carbohydrates, proteins, lipids, nucleic acids are the different macromolecules that are present in…

Q: What is RNA primer?

A: The nucleic acid is a nucleotides chain that stores genetic information. It forms RNA and DNA that…

Q: What is a reporter gene? The product of which reporter geneyields a green color?

A: Reporter gene A gene which is used for locating another part of DNA or gene of our interest is…

Q: What is   transgenesis?

A: A gene is a sequence of nucleotides in RNA or DNA that encodes the synthesis of a gene product,…

Q: What is the difference between eukaryotes and prokaryotes in terms of promoters?

A: A portion of DNA which participates in transcription is called transcription unit. It has three…

Q: How does a promoter dictate which DNA strand is used as the template?

A: The promoter region is referred to as the site where the process of transcription begins on the DNA…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.