Reporter Quencher-absorbs fluorescence given off by reporter when they are close together Oligonucleotide that is complementary to one strand of the PCR product (a) TaqMan probe During the primer annealing step, both a primer and TaqMan probe bind to the template DNA. Forward TaqMan probe primer 5° 3' 3': LulllW. 5' Template DNA that is being amplified During the primer extension step, the probe is digested by Taq polymerase, which separates the reporter from the quencher. Reporter is- not quenched Taq polymerase (b) Use of a TaqMan probe in real-time PCR FIGURE 21.8 An example of a probe, called TaqMan, used in a real-time PCR experiment.

Reporter Quencher-absorbs fluorescence given off by reporter when they are close together Oligonucleotide that is complementary to one strand of the PCR product (a) TaqMan probe During the primer annealing step, both a primer and TaqMan probe bind to the template DNA. Forward TaqMan probe primer 5° 3' 3': LulllW. 5' Template DNA that is being amplified During the primer extension step, the probe is digested by Taq polymerase, which separates the reporter from the quencher. Reporter is- not quenched Taq polymerase (b) Use of a TaqMan probe in real-time PCR FIGURE 21.8 An example of a probe, called TaqMan, used in a real-time PCR experiment.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 15QP

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Select all that apply: Which of these components must be added to a PCR reaction for it to produce a product?O DNA PrimersO Buffers- dNTPsO RNA PrimersO NTPSO PrimaseO Template DNAO Taq DNA Polymerase
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DNA: How PCR works and why it's useful?