WHAT is a scientific question I can ask about Mitochondria & SDH Activity. What hypothesis can I say about malonate, succinate and SDH activity?
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WHAT is a scientific question I can ask about Mitochondria & SDH Activity. What hypothesis can I say about malonate, succinate and SDH activity?
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- Concerning human biology, the major role of a cell's mitochondria is to provide the cells with usable energy in the form of ATP. In regard to the cellular respiration equation: Food + x(consumes) -----> y(produces + energy (ATP). As for "x" (what the cell consumes), I believe the answer is oxygen. As for "y" (what the cell produces), I'm guessing the answer is carbon dioxide. Could you please verify this, and better explain it to me?My question is about cellular respiration. If you test how much CO2 is produced comparing germinating mung beans vs mealworms, what kind of results would you expect to see and why?In the early days of “mitochondriology,” P/O ratios were determined from measurements of volume of O2 taken up by respiring mitochondria and chemical assays for disappearance of inorganic phosphate. Now, however, it is possible to measure P/O ratios simply with a recording oxygen electrode. How might this be done?
- What would be the expected pHs of the matrix and intermembranespace of the mitochondrion? What about the stroma and thylakoidlumen of the chloroplast? Explain your answers.It has been estimated that mitochondria occupy 20% of the volume in the human body. For a 70- kg adult, the average number of mitochondria has been estimated to be 1 × 10 (10,000 trillion). Using this information, provide a rough estimate of the average mass of a mitochondrion.You are isolating mitochondria from insect cells and incubating in a test tube with 0.005 M FADH2, 0.05 M ADP and 0.05 M Pi. Assuming, these 3 can enter mitochondria at no cost,no glucose/products of glucose metabolism remain in the isolated mitochondria, and oxygen is present. Part 1) If all expected reactions go to completion, how much ATP is expected formed? (0 M)(0.02 M) (0.03 M) (0.05 M) (0.1 M) (0.005 M) (0.01 M) (0.015 M) Part 2) The ratio of FADH2/FAD at completion of all expected reactions would be? (2) (>>2) (0) (1) Part 3) After completion of all expected reactions, ratio of H ion concentration inside vs. outside the mitochondrial inner membrane should be? (<1) (~1) (>1)(0) Please provide brief explanation
- Each of the cytochromes in the mitochondrion contains prosthetic groups. What is a prosthetic group? Which type of prosthetic group is associated with the cytochromes?In the early days of “mitochondriology,” P/O ratios were determined frommeasurements of volume of O2 taken up by respiring mitochondria andchemical assays for disappearance of inorganic phosphate. Now, however, itis possible to measure P/O ratios simply with a recording oxygen electrode.How might this be done?Which of the takes place during oxidative phosphorylation in mitochondria? a. protons are pumped from the matrix to the intermembrane space b. protons are pumped from the intermembrane space to the matrix c. electrons are pumped from the matrix to the intermembrane space d. electrons are pumped from the intermembrane space to the matrix e. NADH is pumped from the matrix to the intermembrane space
- Can O2 be considered an uncoupler in the mitochondria to increase electron transport? What about NADH and ADP, Pi?Mitochondria are critical for normal metabolism. From which parent did each human being’s original mitochondria come from at conception? In Luft’s syndrome the mitochondria are not producing sufficient amounts of ATP. What series of reactions could be most responsible for the deficiency? What are some other conditions that are thought to involve mitochondrial malfunction? What's your opinion on the 3-parent babies technique approved in the UK?A culture of the single celled organism Chlamydomonas is actively carrying out photosynthesis in the presence of light and CO2. If you turned off the light, how would you expect the amounts of ribulose 1,5-bisphosphate and 3-phosphoglycerate to change over the next minute? How about if you left the light on and removed the CO2?