What is NOT a way to test the requirement of ylg (your favorite gene) in heart development? OA Knock-down yfg using RNAI OB. Over-express ylg in the heart using a transgene OC. Remove yfg in the heart using Cre/LoxP OD. Cross heterozygous carriers for amorphic yfp mutations together and examine whether 1/4 of them have heart defects OE. Knock-out yfg using CRISPRICAS9
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- Let’s suppose an X-linked gene in mice exists as two alleles, whichwe will call B and b. X-chromosome inactivation, a process inwhich one X chromosome is turned off, occurs in the somatic cellsof female mammals . Allele B encodes an mRNA that is 900 nucleotides long, whereas allele b contains a small deletion that shortens the mRNA to a length of 825 nucleotides.Draw the expected Northern blot that will be obtained usingmRNA isolated from somatic tissue of the followingmice:Lane 1. mRNA from an XbY male mouseLane 2. mRNA from an XbXb female mouseLane 3. mRNA from an XBXb female mouse.Note: The sample taken from the female mouse is not from aclone of cells. It is from a tissue sample, like the one shown.Please type your answer 1A. What is the molecular basis for genomic imprinting? B. How is the process of X-chromosome inactivation similar to genomic imprinting. C. How is the process of X-chromosome inactivation different from genomic imprinting. D. In mice, the Igf2 gene inherited from the mother is never expressed in her offspring? Explain. E. What genes are deleted in a female born with Angelman syndrome. Which parent transmitted the deletion to her?The TBX20 transcription factor is important for the developmentof heart tissue. Deletion of the Tbx20 gene in mice results in poorheart development and the death of mice well before birth. Tobetter understand how TBX20 regulates heart development ata genetic level, Sakabe et al. (2012. Hum. Mol. Genet. 21:2194–2204) performed a transcriptome analysis in which they comparedthe levels of all mRNAs between heart cells from wild-typemice and mice with Tbx20 deleted. This study concluded that TBX20 acts as an activator ofsome genes but a repressor of other genes in cardiac tissue.How might a single transcription factor have oppositeeffects on the transcription of different genes?
- A rare dominant mutation expressed at birth was studiedin humans. Records showed that six cases were discoveredin 40,000 live births. Family histories revealed that in twocases, the mutation was already present in one of the parents.Calculate the spontaneous mutation rate for this mutation.What are some underlying assumptions that may affect ourconclusions?Draw a basket mutant embryo. What does basket encode? Why do the mutant embryos have this phenotype?. The human IGF2 gene is autosomal and maternallyimprinted. Copies of the gene received from themother are not expressed, but copies received fromthe father are expressed. You have found two allelesof this gene that encode two different forms of theIGF2 protein distinguishable by gel electrophoresis.One allele encodes a 60K (Kilodalton) blood protein;the other allele encodes a 50K blood protein. In ananalysis of blood proteins from a couple named Billand Joan, you find only the 60K protein in Joan’sblood and only the 50K protein in Bill’s blood. Youthen look at their children: Jill is producing only the50K protein, while Bill Jr. is producing only the 60Kprotein.a. With these data alone, what can you say about theIGF2 genotype of Bill Sr. and Joan?b. Bill Jr. and a woman named Sara have two children, Pat and Tim. Pat produces only the 60K protein and Tim produces only the 50K protein. Withthe accumulated data, what can you now say aboutthe genotypes of Joan and Bill Sr.?
- A Drosophila embryo dies during early embryogenesis due to arecessive allele of a maternal effect gene called bicoid. The wildtypeallele is designated bicoid +. What are the genotypes and phenotypesof the embryo’s mother and maternal grandparents?In Drosophila, a female fly is heterozygous for three mutations, Bareyes (B), miniature wings (m), and ebony body (e). Note that Bar isa dominant mutation. The fly is crossed to a male with normal eyes,miniature wings, and ebony body. The results of the cross are asfollows.111 miniature29 wild type117 Bar26 Bar, mimatue101 Bar, ebony31 Bar, miniature, ebony35 ebony115 miniature, ebonyInterpret the results of this cross. If you conclude that linkage isinvolved between any of the genes, determine that map distance(sbetween them.Not all inherited traits are determined by nuclear genes (i.e., genes located in the cell nucleus) that are expressed during the life of an individual. In particular, maternal effect genes and mitochondrial DNA are notable exceptions. With these ideas in mind, let’s consider the cloning of a sheep (e.g., Dolly). A. With regard to maternal effect genes, is the phenotype of such a cloned animal determined by the animal that donated the enucleatedegg or by the animal that donated the somatic cell nucleus? Explain.
- Researchers have exploited Minute mutations in orderto study the phenotypes associated with recessive lethal mutations (l−) that decrease the rate of cell divisionand thus make only very tiny homozygous mutant clones that are difficult to analyze. Many differentstrains of Drosophila carry dominant loss-of-functionMinute (M) mutations in a variety of genes encodingribosomal protein subunits. The M genes are haploinsufficient; flies with only one wild-type M+ gene copyhave a slower pace of cell division, and thus prolongeddevelopment and subtle morphological abnormalities.To circumvent the tiny clone problem, researchersgenerate GFP-marked homozygous l−/ l− clones thatare also M+/ M+, in flies that are l−/ l+ and M−/ M+.The loss of the Minute mutation only in cells withinthe clone gives the l−/ l− cells a growth advantageover their neighbors, enabling the mutant clone togrow large enough to study. Diagram chromosomesthat could be used to generate such clonesResearchers know that Fru-M controls male sexualbehavior in Drosophila because inappropriate Fru-Mexpression in females causes them to behave likemales: Such females display male behaviors that areoriented toward other females.a. Describe a fru mutation that could cause the expression of Fru-M in females.b. Describe a transgene construct that scientistscould generate and insert into Drosophila femalesthat would have the same effect as the mutant youdescribed in (a).Are the following events best explained by mutation or epimutation? A. imprinting of the Igf2 geneB. variation in coat color in mice carrying the Avy alleleC. formation of cancer cellsD. variation in flower color between different strains of pea plants,such as purple versus whiteE. X-chromosome inactivation