You are calculating the density of cells (diameter 15 um) in solution using a hemocytometer. You counted201 cells in quadrant 1, 222 in quadrant 2, 217 in quadrant 3, and 216 in quadrant 4. If the volume of eachquadrant is 0.0001 mL, and the dilution factor is 6, what is the concentration of cells per 1 mL of solution?!3!

Hemocytometer is also known as the cell counting chamber, is primarily used for counting cells…

Answered: what is the concentration of cells per…

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Biology

what is the concentration of cells per 1 mL of solution?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.4: The Hemacytometer

Problem 3CP

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Concept explainers

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.

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You are calculating the density of cells (diameter 15 um) in solution using a hemocytometer. You counted
201 cells in quadrant 1, 222 in quadrant 2, 217 in quadrant 3, and 216 in quadrant 4. If the volume of each
quadrant is 0.0001 mL, and the dilution factor is 6, what is the concentration of cells per 1 mL of solution?
!3!

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