What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]?
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What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]?
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- Topic: Multiple Tube Fermentation Questions: 1. What protocol should you follow if gas formation is less than 10%? a. Why not test for pathogens directly instead of usimg indicator organism such as coliform bacteria? b. Can you use the MPN method to test for the presence of coliforms in food? Why or why not? c. Describe another method of determining the presence of coliforms in a water sample.transfer and isolation techniques: What is the reason for flaming the tubes before and after each transfer?Guide Questions: Differentiate the types of culture media based on its physical state and uses. What makes agar good support for microbial growth?
- Activity 13 Urine Culture Inoculating Urine with a calibrated loop The number of microorganisms per milliliter recovered on urine culture can aid in the differential diagnosis of urinary tract infection (UTI). Plastic or wire inoculating loop available commercially, have been calibrated to deliver a known volume of liquid when handled correctly, thus enabling the microbiologist to estimate numbers of organism in the original specimen based on colony forming unit (CFU) of growth on cultures. PROCEDURE: 1st day 1. Gently swirl the specimen bottle to mix the urine specimen. 2. Label all plated media with name of patients, clinical specimen used and date. Label at the bottom of plates and not on the cover. 3. Obtain a disposable calibrated loop. 4. Dip the loop straight into the urine specimen so that the loop part is completely covered. Withdraw straight out. See fig. 16-18 5. Inoculate blood agar plate (BAP) as shown in fig.16-19 6. Incubate at 35 – 37˚C for 18 – 24 hours. 7. Dispose…Catalase Yeast Test Questions: 1. Why is it important to graph volumes that did not include the initial volume? 2. How might the temperature of the yeast mixture affect a reaction? 3. What effect would increasing the amount of yeast have on a reaction?Topic: Pour-Plate Method 2. Molten agar mixed with bacterial suspension has a temperature higher than 45˚CEffect: Rationale:
- TASK 1: Prepare a 0.020 M Cupric Sulfate Solution.Capacity of volumetric flask provided: 50 mLCalculation of mass required: *(MM = 249.68 g/mol) Show how you will prepare this. TASK 2: Preparation of a Dilutions of 0.020 M Cupric SulfateDilution 1 - Prepare a 1:2 dilution of 0.020 M Cupric Sulfate for a final volume of 200 mL.How much stock is required to make this solution?EXPERIMENT 2 Title: Aseptic technique Objectives: To apply the aseptic technique To observe the growth found on the petri dish. Material/apparatus: Gloves, incubator, soap for handwashing, tissues, nutrient agar, 70% alcohol Procedure: Wipe off the bench with 70% alcohol. Draw a line down the middle of the petri dish to divide the plate in half. Label each halves with A and B. Press your unwashed thumb onto the agar at column A. Apply proper handwashing technique. Put the same thumb after washing onto agar at the column B. Incubate the petri dish at 37°C for 24 hours or overnight. Observe and note down the colonies. Results:Subject: Biopharmaceutical technology 1.Identify the types of water that are available and widely used in Biopharmaceuticallaboratory.2. Classify the method in disposing clinical and non-clinical waste.
- General Electrophoresis Questions: 1. What makes macromolecules move through the gel in electrophoresis?2. What determines the speed at which macromolecules move through the gel in electrophoresis? In a single gel, why do some move faster than others?3. Why do we use different procedures for DNA and protein electrophoresis?Paraphrase the text below: Series of test tubes were filled with the desired volume of the BSA (0.1, 0.2, 0.3… 1 ml) .PBS was added to this to make the volume of 1 mL. 5 mL of copper reagent was added to all the tubes. After proper mixing, all the tubes were incubated at room temperature for 15 minutes. 1 mL Folin reagent was added to each and mixed properly with the help of vortex mixer and incubated for 20 minutes. The intensity of the colour was then determined spectrophotometrically at 680 nm. The graph was than plotted between optical density and the amount of BSA. Proteins estimation was done for the detection for the amount of proteins present in the sample solutions by the Lowry methodCOMPUTATIONAL TRUE OR FALSE Milk sample A contained an average of 53 cells per FOV at a dilution factor of 7 whereas Milk sample B contained an average of 99 cells per FOV at a dilution factor of 5. Therefore, milk sample A has less microbial contamination. (Note: 0.01mL of each sample was placed on a clean glass slide and both samples were examined under OIO with an area of 1.77x10-4 cm2.)