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- Blood agar: Selective or differential media? Be able to recognize and/or describe patterns of hemolysis.What kind of cells are suitable for flow cytometric analysis and why? Please answer with 5 dot points/sentences minimumDraw the different types of WBC and state each function. What is the principle behind flow cytometry What is/are purpose of flow cytometry
- how does flow cytometry analyzer works and help in hematologyComplete the dilution series using a maximum amount of 20 mL of dilutent so that the dilution in the final tube is 10^-9Activity 13 Urine Culture Inoculating Urine with a calibrated loop The number of microorganisms per milliliter recovered on urine culture can aid in the differential diagnosis of urinary tract infection (UTI). Plastic or wire inoculating loop available commercially, have been calibrated to deliver a known volume of liquid when handled correctly, thus enabling the microbiologist to estimate numbers of organism in the original specimen based on colony forming unit (CFU) of growth on cultures. PROCEDURE: 1st day 1. Gently swirl the specimen bottle to mix the urine specimen. 2. Label all plated media with name of patients, clinical specimen used and date. Label at the bottom of plates and not on the cover. 3. Obtain a disposable calibrated loop. 4. Dip the loop straight into the urine specimen so that the loop part is completely covered. Withdraw straight out. See fig. 16-18 5. Inoculate blood agar plate (BAP) as shown in fig.16-19 6. Incubate at 35 – 37˚C for 18 – 24 hours. 7. Dispose…
- A flow cytometer is constructed of many components. For good quantitative results how and how frequently should the various systems be calibrated? What is MESF and how does it apply in flow cytometry?EXPERIMENT : CELL COUNTING FOR DETERMINING VIABLE CELL CONCENTRATION Objectives: To count adherent and suspension cell using hemocytometer. To calculate viable cell concentration in a suspension. Procedures: Counting Viable Cells using Hemocytometer/Automated Cell Counter▪ Prepare the hemocytometer slide by cleaning the surface with 70% ethanol carefully (do not scratch the semi-silvered coating). Apply the same step for the coverslip. Wet the edges very lightly and press it down over the grooves and semi- silvered counting area. ▪ Collect 20 μl samples from the cell suspension using a pipettor and transfer it immediately to the edge of the hemocytometer chamber. ▪ Expel the suspension and allow it to be drawn under the coverslip by capillary force. Do not overfill the chamber. Blot off any surplus liquid and place the slide under the microscope. ▪ Alternatively, trypan blue dye can be used to stain cells by mixing an equal volume of trypan blue to a cell suspension (1:1) to…There are three main subsystems to a flow cytometer. Describe the primary function of each one in a short phrase or sentence. Please answer with 9 dot points/sentences minimum