What is the effect of salinity on the rates of photosynthesis versus respiration in Euglena gracilis as shown in Figure 2? What does the difference in the initial slopes of the curves in the left panel tell us? Case Study 2: The researchers used stock cultures of wild-type Euglena gracilis. (a unicellular,freshwater alga). Cells were incubated in the dark in 1.2 mL of 0.1 M potassium phosphate (pH7.4), for determination of oxygen uptake. After approximately 50% of dissolved oxygen hadbeen consumed, a light stimulus was applied to the chamber for determination of oxygenproduction using a Clark-type oxygen electrode. An aliquot of 50 µL of cell suspension wasextracted with 80% acetone and the amounts of Chlorophylls a and b were determinedspectrophotometrically.8040a 200E 60301004020오1002020025050080010002505008001000Light intensity (umol quanta m-2 sLight intensity (umol quanta m-2 s-1Figure 2: Effect of photon flux density (light intensity) on the rate of oxygen production ofEuglena gracilis. The numbers shown on the right side of the graph represent the concentrationof NaCl (mM) added to the growth medium (● = 0 NaCl, O = 100 mM NaCl, A = 200 mMNaCl). The cells were exposed to the indicated light intensities through density filters and therate of oxygen production was measured at 30°C after 2 cycles of respiration/photosynthesis (leftpanel). Thereafter, 10 µM DCMU was added and the rate of respiration was measured (rightpanel). The data shown represent the mean + standard error of 3 different cultures assayed. Inthe right panel, a: P<0.05 (200 vs. 0 NaCl)

Introduction The above graph overall predicts that, for salinities higher than normal, respiration…

What is the effect of salinity on the rates of photosynthesis versus respiration in Euglena gracilis as shown in Figure 2? What does the difference in the initial slopes of the curves in the left panel tell us?

What is the effect of salinity on the rates of photosynthesis versus respiration in Euglena gracilis as shown in Figure 2? What does the difference in the initial slopes of the curves in the left panel tell us?

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Question

What is the effect of salinity on the rates of photosynthesis versus respiration in Euglena gracilis as shown in Figure 2? What does the difference in the initial slopes of the curves in the left panel tell us?

Case Study 2: The researchers used stock cultures of wild-type Euglena gracilis. (a unicellular,
freshwater alga). Cells were incubated in the dark in 1.2 mL of 0.1 M potassium phosphate (pH
7.4), for determination of oxygen uptake. After approximately 50% of dissolved oxygen had
been consumed, a light stimulus was applied to the chamber for determination of oxygen
production using a Clark-type oxygen electrode. An aliquot of 50 µL of cell suspension was
extracted with 80% acetone and the amounts of Chlorophylls a and b were determined
spectrophotometrically.
80
40
a 200
E 60
30
100
40
20
오100
20
200
250
500
800
1000
250
500
800
1000
Light intensity (umol quanta m-2 s
Light intensity (umol quanta m-2 s-1
Figure 2: Effect of photon flux density (light intensity) on the rate of oxygen production of
Euglena gracilis. The numbers shown on the right side of the graph represent the concentration
of NaCl (mM) added to the growth medium (● = 0 NaCl, O = 100 mM NaCl, A = 200 mM
NaCl). The cells were exposed to the indicated light intensities through density filters and the
rate of oxygen production was measured at 30°C after 2 cycles of respiration/photosynthesis (left
panel). Thereafter, 10 µM DCMU was added and the rate of respiration was measured (right
panel). The data shown represent the mean + standard error of 3 different cultures assayed. In
the right panel, a: P<0.05 (200 vs. 0 NaCl)

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