Question 13 What is the effect on the peak corresponding to an acid component when the pH of mobile phase decreases in reverse phase chromatography?

Q: Question 4 What is the factor responsible for the separation in column chromatography? O Polarity…

A: chromatography is based on the mobile phase which is  solvent and stationary phase which is SiO2.

Q: Question- Will the elution contain any activity? After running an ion exchange chromotograpraphy…

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Q: [2] How might a better chromatographic separation of solutes be accomplished? [3] What would happen…

A: Chromatography is one of the most important techniques that is used analytical techniques. It is…

Q: What is the basic principle for Thin layer chromatography?

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Q: How does the polarity of the mobile phase affect chromatograms?

A: Chromatograms are the pattern formed on an adsorbent medium by the layers of components separated by…

Q: two general methods for improving the resolition of two  substances on chromatography column

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Q: In chalk chromatogaphy experiment, what makes the separation of the components of ink possible?

A: Chromatography contains two fundamental components: a mobile phase, which is basically the analyte…

Q: In choosing a mobile phase for chromatography, which characteristic is preferred? a. Volatile b.…

A: Let us discuss mobile phase and stationary phases of chromatography.

Q: Silica gel column chromatography of biphenyl used 4:1 hexane:ethyl acetate as the solvent. If it…

A: In silica gel chromatography, the silica gel is the stationory phase and  a volitle organic solvent…

Q: For ion exchange chromatography, elution step is used to unbound components to obtain the desired…

A: Ion-exchange chromatography is an important analytical technique which is based on the attraction…

Q: Question attached

A: TLC It is abbreviated as thin layer chromatography. It is a technique used to isolate non-volatile…

Q: Which is the correct order of elution of a gas chromatography that has a polar column that contains…

A: The sample, which may be a gas or a liquid, is injected into a gaseous mobile phase during gas…

Q: a. What does ECD mean. b. Discuss how column temperature influences separation in a chromatography…

A: “Since you have posted a question with multiple sub-parts, we will solve the first three subparts…

Q: Explain about Liquid Chromatography technique.

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Q: . What is lon chromatography?

A: chromatography  : Chromatography can be defined as the chromatography is a physical technique of…

Q: In thin-layer chromatography, what is the Rf value of a compound if the solute travels 2.9 cm and…

A: Retention factor: The ratio of distance traveled by the substance to the solvent is known as the…

Q: a. Calculate the retention factor for each spot from lanes A to E . b. Which ink standard was the…

A: Paper Chromatography is a technique where paper is used as the stationary phase through which the…

Q: Question 12 Which of the following is not true about High pressure liquid chromatography (HPLC)? A…

A: High Performance Liquid Chromatography (HPLC) is one of the most versatile tool used in analytical…

Q: Analysis of an unknown plant extract yield two peaks X and Y in a chromatogram, 15.65 and 17.03 min…

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Q: Analysis of an unknown plant extract yield two peaks X and Y in a chromatogram, 15.65 and 17.03 min…

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Q: Question 10 Column chromatography is based on the principle of lon-exchange Exclusion principle…

A: Column chromatography is used to seperate components of solute based on the difference in polarity…

Q: (6)(Inorganic) In an ion exchange chromatography column, based on sulfonate groups, as shown in…

A: The anions are produced by the removal of hydrogen ion (H+) from a neutral compound such as…

Q: Which of the following changes would work to improve chromatographic separations? Explain briefly…

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Q: You are monitoring the reaction A --> B via periodic GC analysis. Initially, you only see one peak…

A: GC analysis is analytical technique by which separation of chemical mixture into its constituents…

Q: Three compnent a,b & c seperate by use thin layer chromatography according to following figure, What…

A: Rf value or the Retention factor value signifies the distance a component travels relative to the…

Q: What is meant by retention time in gas chromatography.  In what order will they come hexane, ethyl…

A: The process of separation of various constituent of a mixture in zone or in phases is known as…

Q: Discuss the basic principles behind cation chromatography technique

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Q: What is reverse-phase ion-pairing chromatography?

A: This is an more efficient Technique in which counter ions of solute particles are used .

Q: What is the principle behind reverse-phase ion-pairing chromatography?

A: Reverse Phase ion chromatography use the principle of  Solute ions instead of molecules .

Q: What is the major driving force in chromatographic separation? difference in affinity with a…

A: Chromatography has been defined  primarily a separation process which is used for the separation of…

Q: Which Is The Most Commonly Used Detector In High Performance Liquid Chromatography And Why

A: High Performance Liquid Chromatography is abbreviated as HPLC. High performance liquid…

Q: Which of the following is TRUE for a reversed phase chromatography experiment?    i. Less polar…

A: Reversed phase chromatography is the chromatographic technique where stationery phase is non-polar…

Q: What is the difference between an ascending and descending paper chromatography?

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Q: State 2 properties of detectors used in chromatography?

A: A detector used in chromatography is basically used to detect the components of mixture which is…

Q: What are the quantification methods for supercritical fluid chromatography?

A: Interpretation- To give all the quantification methods for supercritical fluid chromatography -…

Q: A chromatography column with a length of 10.3 cm and inner diameter of 4.61 mm is packed with a…

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Q: What is Jon chromatographys

A: Given To know about the chromatography

Q: What is the role of stationary phase and mobile phase in Chromatography?  Please shortly answer at…

A: The stationary phase remains fixed in place while the mobile phase carries the components of the…

Q: Name two general methods for improving the resolution of two substances chromatographic column.

A: Good resolution columns provide a great separation of the components by a column chromatography.…

Q: is the chromatography in reverse or normal phase if the standards and the sample are spotted in a…

A: The given type of paper chromatography is normal phase or reverse phase explanation is given below…

Q: An ion chromatograph is used for the determination of chloride ions. Seven replicate asurements of…

A: Given: Number of replicates  = 7 The replicate measurements obtained are 20, 21, 18, 22, 20, 22, and…

Q: thin layer chromatography What would happen to it if you decreased the polarity of the mobile…

A: Thin Layer Chromatography is used to monitor the progress of the reaction. It is composed of the…

Q: What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly…

A: TLC

Q: Ion exchange chromatography can be used to separate a mixture of cations and anions, true or false?

A: Chromatography is the method used in laboratories for the separation of elements or molecules.

Q: thin layer chromatography: What would happen to the Rf value of a compound if you increased the…

A: One of the methods of identification of the individual compounds from a mixture of compounds is TLC.…

Q: State the major differences among liquid,gas and super critical fluid chromatography?

A: Super critical fluid is intermediate between liquid and gas.

Q: what is the effect on the chromatographic peak if a. inserting/adding sample too slow b. column…

A: Effect of chromatographic peak on  a. inserting/adding sample too slow b. column temperature is too…

Q: Wrtite a brief note on various operations involved in paper chromatography.

A: Chromatography is a qualitative and quantitative type of separation technique in which the mixtures…