Q: what are advantages and disadvantages of using STRmix software in degraded DNA and DNA mixtures?
A:
Q: What causes different linear DNA molecules to migrate at different speeds during electrophoresis?
A: In molecular biology many techniques are used for different purpose like PCR is used to generate…
Q: How does size of DNA affect size traveled during electrophoresis?
A: Electrophoresis is a technique for the separation followed by the analysis of the macromolecules…
Q: Is the DNA isolated from Cheek Cells using Saline solution, Detergent, and Ice-cold alcohol pure or…
A: DNA is a genetic material present in every cell.
Q: Why does DNA moves towards anode in gel electrophoresis?
A: Gel electrophoresis is process that separates the molecule usually protein or DNA on the basis of…
Q: In gel electrophoresis, are the DNA fragments attracted to, or repelled from the negative pole of…
A: Electrophoresis is a process where charged particles move under the influence of the electric field.…
Q: What benefits are there when using a DNA microarray over a genetic marker such as a STR?
A: The term genetic refers to the DNA molecule present in the nucleus of cells. DNA or deoxyribonucleic…
Q: What is the mom method of pacaking DNA?
A: Answer: DNA : Deoxyribonuleic Acid acts as the genetic material which transfers the genetic…
Q: In DNA extracting. What is the purpose of clear shampoo in the DNA extraction buffer?
A: DNA is extracted from different sources to analyze and study the DNA sequence and diseases caused…
Q: When proteins are separated using native gel electrophoresis, size, shape, and charge control their…
A: Gel electrophoresis is a method for separation and analysis of macromolecules like DNA, RNA, and…
Q: Describe the automated dideoxy chain termination method of DNA sequencing.
A: The chain termination DNA sequencing method is the Sangers sequencing in which the DNA fragment is…
Q: Describe the processes involved in denaturing and renaturing of DNA, and explain what is useful…
A: DNA stands for deoxyribonucleic acid. It is the genetic material of the organisms that transfer from…
Q: How does magnetic bead DNA extraction work?
A: DNA extraction is the method of isolating DNA by disrupting the cell wall and nuclear membrane with…
Q: Explain the principles of DNA migration of sample A in the electrophoresis experiment
A: Electrophoresis is a separation technique that was used to separate the mixture of molecules from a…
Q: How does a DNA microarray work?
A: Gene microarray technology enables to deposit many different DNA sequences on a small glass slide,…
Q: How do we convert RNA to DNA in RT-PCR?
A: Introduction:- The hereditary ingredient in the cell is genetic material. It contains all of an…
Q: What is the useful of the Development of highly sensitive and low-cost DNA agarose gel…
A: In today's medicine, extremely sensitive and low-cost DNA agarose gel electrophoresis is widely used…
Q: What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
A: Deoxyribonucleic acid (DNA) is the hereditary unit of life, which carries the genetic information in…
Q: Why is the annealing temperature different between samples during DNA extraction & marker gene…
A: Introduction Polymerase chain reaction or PCR laboratort technique by which millions to billions of…
Q: Is the DNA extracted from banana pure? Explain
A: DNA (deoxyribonucleic acid) is the genetic material in all living organisms. DNA exists as a double…
Q: Describe capillary electrophoresis. How does this technique distinguish between input DNA and…
A: Capillary electrophoresis is an electrophoretic technique used in analysis of compounds to separate…
Q: what type of gel(in terms of material) can be more suitable for the electrophoresis of 500-1000bp…
A: DNA tests are stacked into spaces toward one side of a gel, and an electric current is applied to…
Q: What are nanopores used for and tell How does DNA bind to the nanopore?
A: Deoxyribonucleic acid (DNA) sequencing is the process of determining the nucleic acid sequence in…
Q: What is cell lysis? Discuss the principles behind DNA isolation (extraction and precipitation…
A: A eukaryotic cell consists of many membrane-bound organelles. The membrane-bound organelles form…
Q: What is oligonucleotide microarray?
A: An array is an orderly arrangement of samples where matching of known and unknown DNA samples is…
Q: What are possible mistakes that can cause the DNA to be contaminated during isolation, purification…
A: Various techniques are used to extract different types of DNA. Cells are broken open using a…
Q: How does gel electrophoresis separate DNA fragments of your unknown sample and How will you be able…
A:
Q: What information can be gathered by using DNA gel electrophoresis? Describe the steps in the process…
A: Gel electrophoresis is a molecular biology technique heavily used in laboratories. Electrophoresis…
Q: what is the Jusage of DNA separation in gel electrophoresis
A: DNA is abbreviated as deoxyribonucleic acid, which is known as hereditary material in most organisms…
Q: In the alkaline plasmid screen, what two things separate plasmid from chromosomal DNAs?
A: Following is the procedure for the separation of plasmid by the alkaline lysis method.
Q: Is it possible to obtain DNA in cloverleaf configuration? Explain
A: Cloverleaf Model: The cloverleaf model of tRNA is a representation of the molecule's structural…
Q: What is the Ti plasmid and how has it been of use ingenetic engineering?
A: A genetically engineered organism is the result of recombinant DNA technology that involves the…
Q: How do we separate plasmid and chromosomal DNA during the alkaline plasmid screen?
A: Alkaline lysis is the most common method used for separating plasmid DNA from chromosomal DNA.
Q: What are restrictionenzymes? How do theseenzymes participate in therecombinant DNA technology?
A: Recombinant DNA technology involves integrating DNA molecules from two different species and…
Q: What is used to identify the size of a DNA molecule during gel electrophoresis?
A:
Q: Explain the limitation of DNA microarrays ?
A: DNA microarray is the molecular laboratory technique that helps in studying and measuring the…
Q: What are the principles of Chelex extraction and solid-phase DNA extraction methods?
A: To explain the principles of Chelex extraction and solid-phase DNA extraction methods The principle…
Q: What is DNA fingerprinting? Define and differentiate VNTR and Probe and what is the applications of…
A: Step 1 DNA or deoxyribonucleic acid occurs in living systems and can function as genetic materials.…
Q: What does A230 indicate, in assessing extracted DNA quality?
A: Option C Extraction contaminants
Q: Gel Electrophoresis Separates DNAFragments According to which thing?
A: Electrophoresis is a technique used for separating the components of a mixture of charged molecules…
Q: What are the functions of Glacial acetic acid and potassium acetate on plasmid DNA analysis
A: Plasmid isolation uses many salts and detergent for gradual separation of DNA from proteins and cell…
Q: What compounds remain in the ethanol solution after the DNA is removed bt spooling onto a glass rod?
A: Introduction: The extraction of DNA is the first step for subsequent molecular or forensic…
Q: why does a higher agarose concentration render better resolution/separation of smaller DNA…
A: Electrophoresis is an essential lab procedure at the molecular level that uses an electric field to…
Q: How does a DNA microarray work amd How do you make a DNA microarray?
A: DNA microarray is a collection of DNA sequences attached to a solid surface such as a glass slide…
Q: Aside from gel electrophoresis Give another method to quantify DNA. Explain the concept behind this…
A: DNA quantification is a type of quantification of nucleic acids that is used for the determination…
Q: What is a short tandem repeat (STR) and which method do we use to detect it and which part of the…
A: Short-term repetition of tandem (STRs) is a short-lived sequence of DNA (2–6 bp) that accounts for…
Q: Explain why we choose to use 1% agarose gel for Electrophoretic Analysis of DNA via Agarose gel…
A: Answer: Agarose Gel Electrophoresis : It is the gel electrophoresis technique which is used to…
Q: Explain why DNA fragments migrate in a gel electrophoresis. Which fragments migrate farthest: large…
A: Introduction :- The technique of gel electrophoresis is used to separate DNA fragments based on…
Q: how can denaturing environment be created during electrophoresis? which bonds are broken during DNA…
A: Definitions: Electrophoresis: Electrophoresis is defined as a laboratory technique that involves the…
What is the mechanism of separation of large DNA fragments in pulsed field gel electrophoresis.
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- why does a higher agarose concentration render better resolution/separation of smaller DNA fragments? and what determines the distance of DNA fragments in gel electrophoresis?What is the cause of an extra band in a DNA ladder in agarose gel electrophoresis?How does gel electrophoresis separate DNA fragments of your unknown sample and How will you be able to track the distance travelled by the DNA during electrophoretic run?
- Why is the annealing temperature different between samples during DNA extraction & marker gene amplification? How is this computed?What is used to identify the size of a DNA molecule during gel electrophoresis?How are DNA molecules visualized in a gel after electrophoresis? Why do DNA molecules migrate toward the + electrode? What determines the rate of their migration? What is the effect of PEG on DNA fragments of different sizes? How is this influenced by the concentration of PEG?