In DNA extracting. What is the purpose of clear shampoo in the DNA extraction buffer?
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What is the purpose of clear shampoo in the DNA extraction buffer?
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- Which Strict Operating Requirements DNA Polymerase Has?During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityExplain the Doyle and Doyle (1990) method of extraction. Identify which step removes specific macromolecules and how they do so until you reach DNA purification.
- In DNA purification, explain how the chromosomal DNA is separated from the plasmid DNA? Be sure to mention the specific buffer components that facilitate this process.Replication:- what other enzymes are involved in the initiation phase?- explain the role of primers in this phase- how is the building of the leading strand different from that of the lagging strand?proteins are denatured and eliminated during the DNA extraction process. how can we determine the purity of our DNA sample?
- Why do we need to add “COLD” alcohol? What is the effect of the temperature of the alcohol in the process of extracting the DNA.Why is DNA not soluble in ethanol whereas other components cells are soluble. What is the rationale behind DNA precipitation in solutionWhat additive in the agarose gel solution allows the DNA to fluoresce (glow) under UV light? How does this work?
- Why DNA melting is required in PCR? Briefly explain how PCR can be used to detect DNA mutation.List essential components required for the DNA sequencing reaction based on the Sanger method. Briefly describe the purpose of each componentAnalyzing Cloned Sequences What kind of information can a DNA sequence provide to a researcher studying a disease-causing gene?
