What is the purpose of a bacterial smear? What is the purpose of a bacterial smear? It spreads the bacteria out into a single layer. It kills the bacteria. It helps the bacteria stick to the slide so they do not wash off during staining. All of the above. None of the above.
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What is the purpose of a bacterial smear?
What is the purpose of a bacterial smear?
It spreads the bacteria out into a single layer.
It kills the bacteria.
It helps the bacteria stick to the slide so they do not wash off during staining.
All of the above.
None of the above.
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- Which of the following are true about a differential stain. There may be more than one answer! No chemicals are needed. More than one stain is used The acid fast stain is an example The Gram stain is an example.The first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stainThe first step in identifying an unknown bacterium is to __________. The first step in identifying an unknown bacterium is to __________. culture your unknown on selective media perform endospore staining on your culture prepare a pure working stock simple staining on your culture acid-fast staining on your culture
- Why is it essential that smears be air-dried? Why can’t they be gently heated over a flame to speed up the drying process?Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the following usually needs to happen (and why)? Staining (to increase magnification). Heat, radiation or antimicrobial chemical treatment (to kill the bacteria for safe observation). Add immersion oil (to increase resolution). Add a cover slip (to reduce contamination). The viewing chamber needs to be flushed of air (to create a vacuum).In spread plate method, inoculating loop is used to spread bacteria from the inoculation site. For quadrant streaking, the loop must be flame sterilized between quadrant streaks. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.
- Which stain is used 1st after you heat fix the fly during the gram staining procedureYou can perform a specific staining method to confirm the purity on the culture. Describethis staining method.Which of the following can be used to distinguish one colony from another on an agar plate? Select all that apply. a. Shape b. Color c. Gram stain d. Cell arrangement e. Size f. Elevation g. Margin h. Texture
- name 5 things about method staining.You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point? ○ pink ○ colorless ○ blue ○ purpleThe acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______. Positive cells that retain the primary stain are ______, while negative cells are counterstained with _____ and will have a ______ color. *Please use answers from the options provided in the image below.