The first step in identifying an unknown bacterium is to __________. culture your unknown on selective media perform endospore staining on your culture prepare a pure working stock simple staining on your culture acid-fast staining on your culture
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| culture your unknown on selective media |
| perform endospore staining on your culture |
| prepare a pure working stock |
| simple staining on your culture |
| acid-fast staining on your culture |
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- Is is best to observe slides of bacteria stained with the acid fast stain under high dry magnification. True or falseThe first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stainAfter performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple bacteria. Which of the following could notexplain why this occurred? Decolorizer was used after safranin Safranin was not used Crystal violet was not used The specimen was not heat-fixed
- Explain how bacterial cells would look in the gram staining procedure if the following mistakes were made: a. Decolorizer left on too long b. Decolorizer not left on long enough c. Slide not heat-fixed before stainingThe unknown observations are shown as a mixed stain, gram positive, and gram negative. The next images shows the chart of what we can choose from for the gram positive bacterial stain. We are allowed to choose 4 test or stains that we would like to use to preform to identify the unknown.Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.
- observe the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram staining c. Negative staining d Direct staining eAll the aboveThe presence of a capsule around bacterial cells usually indi-cates their increased disease-causing potential and resistance todisinfection. Capsules are generally viewed by:(a) Spore staining(b) Scanning electron microscopy(c) Gram staining(d) Ziehl-Neelsen staining(e) Negative staininga pure bacterial culture was diluted by adding a 0.2 mL aliquot to 0.9mL water. Then 0.1 mL of this dilution was plated out, yielding 82 colonies. Calculate the CFU/mL in the original culture.
- In spread plate method, inoculating loop is used to spread bacteria from the inoculation site. For quadrant streaking, the loop must be flame sterilized between quadrant streaks. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.When you interpret a Gram-stained smear, you should also describe the morphology (shape) of the cells, and their arrangement. In the figure below, there are two distinct types of bacteria, distinguishable by Gram stain reaction, and also by their shape and arrangement. Below, describe these characteristics for both bacteria: Gram positive bacterium Gram negative bacterium Morphology cocci bacillus ArrangementThe below photograph shows a Gram-stained slide viewed using a light microscope set at brightfield of a clinical sample from a patient with symptoms suggesting an infection. The slide was viewed with x100 objective. What could be the most likely cause of infection? Gram positive bacteria, either rods or cocci Gram positive rod shaped bacteria or yeast Gram negative rod or cocci shaped bacteria Gram negative bacteria, either rods or cocci