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What is the purpose of flaming an inoculating loop?
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- Why is it necessary to inoculate the KIA medium all the way down to the bottom of the tube during inoculation?What is the hazard of the splattering tendency in flame sterilizing an inoculating loop? Can inoculation loops and needles be sterilized by autoclaving? Explain When is sterilization by filtration preferred over sterilization by heat? Why?How does an autoclave sterilize instruments? What is used to dry glassware and at the same time sterilize?
- Why is it important to always flame the mouth of a test tube or a petri plate in performing aseptic transfer techniques. In flaming a wire loop, why does it need to be red hot?What is the point of the pressure in the autoclave sterilization?What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter culture media for a concentration of 6.5 × 106 cells/mL?