What is the purpose of flaming an inoculating loop?
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Q: what is the purpose of flaming an inoculating loop?
A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…
Q: How does an autoclave sterilize instruments? What is used to dry glassware and at the same time
A: Autoclaving is the best method for sterilizing glass instruments. Sterilizing means removal of all…
Q: Why del factor is important in media sterilization?
A: Sterilization, which was called by various means such as Del factor, Nabla factor and the…
Q: Under what conditions are media typically sterilized in an autoclave?
A: An autoclave is the pressure chamber used to carry out the scientific and industrial processes…
Q: How is the CTSA (Coconut tryptic soy agar) growth medium designed to meet the nutritional need of…
A: Coconut trypic soy agar media are general-purpose, non-selective media that provide enough nutrients…
Q: Define the following terms; Sterilization, and Aseptic Technique. What are the differences between…
A: Microbes invading and proliferating within an organism's body causes infection. Microbes include…
Q: What is the point of the pressure in the autoclave sterilization?
A: Autoclaves are the devices which are like pressure cookers and used in laboratories that provide a…
Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…
A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…
Q: Explain the steps of preparing a cell culture stock from a T25 flask.
A: Cell culture is the specialized process used in molecular biology to remove cells from plants and/or…
Q: What is diluent? Why is it useful for working with bacterial samples?
A: Dilution is done to decrease the concentration of the original sample that is being tested. Dilution…
Q: How is the CTSA (coconut water tryptic soy agar) growth medium designed to meet the nutritional need…
A: Many different kinds of media are formulated in the microbiology lab to support the growth of a wide…
Q: What conditions are necessary to destroy endospores? In what device are these conditions achieved?
A: Endospores are dormant vegetative cells that have a thick outer covering making them resistant to…
Q: What conditions are necessary to destroy endospores? In what device are these conditions achieved?
A: The endospores are the non-reproductive, dormant, tough structure produced by archaea (firmicutes)…
Q: What is the basic principle of GPC (gel permeation chromatography)
A: Gel permeation chromatography is a simple way to determine the molecular weight of your sample…
Q: Why does a precipitate form on Sierra’s medium when inoculated with a lipolytic organism? Discuss…
A: Several nutrient mediums are used in order to inoculate several organisms for experiments.
Q: What is the similarities of differential centrifugation and gradient centrifugation?
A: Similarities between differential centrifugation and gradient centrifugation is process involved…
Q: Why are aseptic techniques implemented during inoculation?
A: Aseptic means without contamination and Inoculation means transfer of a bacterial sample onto a…
Q: Why do you think autoclave sterilization of media is preferred over open flame?
A: An autoclave is used to sterilize surgical equipment, laboratory instruments, media and other…
Q: What is the volume of broth that can be picked up by loopful of sterile inoculating loop?
A: INOCULATING LOOP It is a simple tool mainly used to pick up and transfer a small sample from a…
Q: Why is CFU more applicable to a culture of Streptococcus than to a culture of E. coli?
A: Colony-forming unit (CFU) represents the number of colonies formed on the media plate from one…
Q: Why are plated media stored in an inverted position?
A: Plated media enables the isolation of microorganisms from samples or specimens.
Q: What will pose an ethical issue in these procedures are performed - sterilization?
A: Sterilization is a permanent method of family planning. It is one of the most common method of…
Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…
A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…
Q: Write the limitations of sterilization by Dry heat?
A: The dry heat sterilization technique includes the removal or killing the microorganism and require…
Q: What is the principle behind invertase test
A: Invertase was an enzyme that hydrolyses the disaccharide sucrose into monosaccharides glucose and…
Q: what is sterilization or define sterilization?
A: Sterilization is defined as the process where all the living microorganisms, including bacterial…
Q: What is the structural and functional differences between Inoculating loop versus inoculating…
A: Inoculating tools These tools are use by microbiologist to inoculate microorganisms easier.
Q: What settings and time are normally used for sterilization in an autoclave?
A: The fundamental rule of steam sterilisation as cultivated in an autoclave, is to open every thing to…
Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…
A: Smear - The word smear defined as the process to spread the stick substance or solution on the…
Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…
A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…
Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…
A: The smear preparation is an important technique that is followed in microbial laboratories. The main…
Q: Contrast the terms thermal death time and decimalreduction time. How would the presence of…
A: Microbial growth can often be controlled by simple methods such as disinfection and decontamination.…
Q: Why is the sterilization important in healthcare system and can you give briefly explain?
A: Medical sterilization mainly makes use of three important terms such as sterilization, disinfection…
Q: Inoculating with 8 S.aureus has a generation time of 60 minutes how many bacteria after 6 hours?
A: Exponential (log) Growth Phase- The exponential phase of growth is a process of balanced growth in…
Q: What is the principle of freezing of cell lines and primary cells? Describe the general protocol for…
A: Cryopreservation is the method of keeping the cells, tissues and other biological samples in a deep…
Q: What type of media components cannot be sterilized in an autoclave?
A: Sterilization refers to the complete destruction or elimination of all viable organisms. The process…
Q: What is the effect of trypsin EDTA solution on monolayer cell culture preparation?
A: Trypsin is an enzyme which helps in digestion of proteins.In the small intestine,proteins are broken…
Q: what is the cause of different species of psychrotrophic bacteria growing in the CTSA medium at 7…
A: They can grow at extreme low temperatures and lead to spoilage of food by modifying the compounds…
Q: What is the advantage of the plating method over an electronic cell counting method in counting…
A: Bacteria culturing is done in order to grow the bacterial strains that are able to show resistance…
Q: If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock…
A: Given: volume of stock solution is 15 mg/100 ml Needed amount is 1 mg kinetin in 25 ml Volume of…
Q: Are you able to identify the lag, log, stationary and death phase in using this growth curve? Are…
A: The growth curve of bacterial cells can be divided into four distinct phases known as lag, log (or…
Q: What is the purpose of flaming an inoculating loop? How will you know when you have flamed the loop…
A: Hi, since you have posted multiple questions, we will be providing you with answers for the first…
Q: Explain Ricin is a antineoplastic natural products?
A: Ricinus communis L. (castor plant) though originating in Africa, it is found worldwide and is grown…
Q: Why is Hydrogen peroxide solution so effective against anaerobes?
A: Hydrogen peroxide is a chemical compound that is acts as mild antiseptic. It is also an endogenous…
Q: What process would explain why the dyes spread through the agar?
A: Agar -- Agar is polymer and mixture of polysaccharides obtained from the cell wall of red algae .…
Q: Which piece of equipment sterilizes hospital tools and devices and how does it sterilize things? Are…
A: Sterilizing pieces of equipment are very important in modern healthcare settings. It is necessary…
Q: A Pure colony is needed for inoculation in sterile water, a small portion of culture media was…
A: Introduction Culture is referred to the process of growing the cell outside the animal or plant…
Q: In preparing bacterial smear, why do we need to pass the slide over the flame?
A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…
Q: Why does the nitrate reduction tube turn red after the addition of zinc?
A: Nitrate reduction test is used to find the bacteria who have the ability to reduce the nitrate.
Q: What is the most common sterilization technique used in laboratories?
A: Sterilization alludes to any process that removes, kills, or deactivates all various forms of life…