What is the structural and functional differences between Inoculating loop versus inoculating needle?

Q: Why is the order of separation based on size opposite for gel filtration and gel electrophoresis,…

A: Gel filtration and gel electrophoresis are biochemical techniques used for the separation of…

Q: What is ninhydrin used to detect? How does it work?

A: Proteins are involved in several important body functions and are very much essential for all life…

Q: What is indirect immunofluorescence microscopy?

A: BASIC INFORMATION MICROSCOPY  It is a field in which the microscope used in order to objects which…

Q: Provide the function of the following in blotting techniques: Nitrocellulose paper  paper weight…

A: Function of Nitrocellulose paper in blotting:  • Nitrocellulose paper is used in protein blotting…

Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…

A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…

Q: What are the pros and cons of serial dilution method and W/V % method?

A:

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: explain why it is not good to break sterile field  when inserting a foley catheter

A: Inserting the indwelling catheter is an invasive procedure. Sterile technique should follow while…

Q: Aside from observing motility, what are the other uses of the wet mount and the hanging drop…

A: Introduction:  A wet mount slide is one that has a liquid in the center that will act as a medium…

Q: what molecules can prevent anabolic steriods form working?

A: It acts by promoting nitrogen retention in muscles, leading to an increase in muscle size, and…

Q: to observe the motility of an UNKNOWN Bacteria isolated from a mixed culture, which technique/s is…

A: Motility is the ability of an organism to move from one place to other, generally, the bacteria…

Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…

A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…

Q: How will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?

A: Answer:  Rapid Strep A Test (Rapid strep test) is a test used to identify the presence of Strep A…

Q: What is MODIFIED ALLEN TEST?

A: Blood is an important fluid tissue that is used to transport nutrients and oxygen to all parts of…

Q: What is the reason for using sterilized filter paper in CHORIOALLANTOIC MEMBRANE (CAM) Assay?

A: The formation of new blood vessels from pre-existing vessels or endothelial cell progenitors is…

Q: What is the differece between disinfection and sterilization? What is the dfference between…

A: Disinfection and sterilization are essential for ensuring that medical and surgical instruments do…

Q: What are the advantages and disadvantages of the viable plate count method?

A: Microbiology is the branch of science that studies microscopic organisms. As microorganisms are not…

Q: How does gel filtration work? What are the applications of analytical versus preparative versions…

A: Chromatography is a technique for separating a mixture in the laboratory. The mixture is dissolved…

Q: What is the procedure of beta method?

A: Beta-lactamase test is used for the detection of enzyme beta-lactamase. This enzyme provides the…

Q: What other method could be used to purify lysozyme from white using its unusual pl?

A: The unique charge properties of lysozyme with a very high pI of about 10.5 can be used in the…

Q: what is the diffrence between Aseptic technique and sterile technique

A: Aseptic technique Also called as asepsis. It means absence of germs such as bacteria, viruses, and…

Q: What side of the electrophoresis chamber (red or black) should the wells be on when the gel is…

A: The agarose gel electrophoresis is a technique that is used to separate DNA fragments as per their…

Q: what are the important principle in invertase test?

A: To determine enzymatic activity, the amount of substrate remaining or the amount of product created…

Q: How does plating efficiency affect the number of plaque-forming units? How is plating efficiency…

A: A viral plaque is defined as the visible structure formed on the culture plate of bacteria or any…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: What is the basis of determining a microorganism in being susceptible or resistant to a specific…

A: Microorganisms are tiny living creatures that are not visualized by the naked eye. They should be…

Q: What is the effect of trypsin EDTA solution on monolayer cell culture preparation?

A: Trypsin is an enzyme which helps in digestion of proteins.In the small intestine,proteins are broken…

Q: What is the principle behind invertase test

A: Invertase was an enzyme that hydrolyses the disaccharide sucrose into monosaccharides glucose and…

Q: How do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect?…

A: Several tests are performed in the laboratory in order to detect several bacterial agents as well as…

Q: When using the blue/ white colony screening method, what do blue colonies represent ?

A: Blue- white screening is used for the identification of recombinant bacteria.

Q: Why differential centrifugation can cause contamination in pellet?

A: Differential centrifugation is a typical process used in microbiology and cytology to separate…

Q: In performing the Kirby-Bauer procedure in a clinical laboratory setting, why must the agar be a…

A: The Kirby Bauer antibiotic test, also known as the disc-diffusion test, employs an antibiotic disc…

Q: What is the advantage of cryo-sectioning for antibody staining methods?

A: To study the antigenic structures, present on the cell surface tissues can be embedded in paraffin…

Q: What is the coliform test when is it used? What is its purpose? How does it function? What are the…

A: All water that is naturally exposed to the atmosphere, such as rivers, lakes, and reservoirs. It is…

Q: What are the key considerations for the scale-up of cell culture process for the large molecule drug…

A: Well developed skills are needed to increase the batch yield and to reduce processing time for large…

Q: What is the relationship between the zone of inhibition and antibiotic concentration and what is the…

A: The zone of inhibition is a circular area around the spot of the antibiotic in which the bacteria…

Q: E. coli O157:H7 is an organism of concern in contaminated foods. How can the use of MacConkey agar…

A: MacConkeys Agar is a selective and differential culture medium. It specifically select Gram negative…

Q: the chemicals used in the gram staining method?

A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram staining…

Q: Define the following terms; Sterilization, and Aseptic Technique. What are the differences between…

A: Microbes invading and proliferating within an organism's body causes infection. Microbes include…

Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…

A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…

Q: You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is…

A: The dilution factor may be expressed as the ratio of the concentration of stock solution to the…

Q: Discuss the functions of the following components in performing gel electrophoresis;…

A: Introduction: The technique of gel electrophoresis is used to separate DNA fragments based on their…

Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?

A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…

Q: How are dilution susceptibility tests and disc diffusion tests usedto determine microbial drug…

A: Although not all infectious agents require antimicrobial sensitivity testing, it is essential in…

Q: What are the best characteristics of an antibiotic to be used for antibiotic susceptibility test?

A: Antibiotics are the class of drugs that show antibacterial property against certain strains of…

Q: Why are aseptic techniques implemented during inoculation?

A: Aseptic means without contamination and Inoculation means transfer of a bacterial sample onto a…

Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…

A:

Q: What is the mechanism of an antibiotic removal device (ARD)?

A: Blood cultures are prepared to detect the presence of a pathogenic microbe in the blood. If the…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.