What is the role of the plasmid ? Explain the experimental details of the transformation. How can the success of transformation be observed?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Restriction Enzymes Handout
plasmid
Restriction Enzymes
Bam HI
Eco RI
Hpa I
Hind III
Nde I
Please solve this question in less than 15 minutes for genetics engineering
Sal I
Plasmid Handout
DNA Sequence
(both strands are represented)
GIGATCC
CCTAG G
U
G|AATTC
CTTAA G
GTT | AAC
CAAjTTG
A|AGCTT
TTCGA | A
CATATG
GTATAC
G|TCGAC
CAGCT | G
agtgacata tga ttcgag c cggta a c
tca ctgtat a ст aagctcgagccattg
cggggat cctctag agtcgacctgcagg c
gcccctaggagatctcagctgga cgtc cg
tagca ag c tggcgt at atggta cata
at cgttcga accgcattagtaccat gta t
gggatceptet ct ccagtaggtaggc cgtcg
ccct agga aga g g t catccat
cc ggcag c
Antibioty
Resistance gene
gc taggetta a actgggatccat gcc
origin o
tplgm cg at cc a at ttgaccctaggta cgplasmid
replication
What is the role of the plasmid ? Explain the
experimental details of the transformation. How can the
success of transformation be observed ?
Transcribed Image Text:Restriction Enzymes Handout plasmid Restriction Enzymes Bam HI Eco RI Hpa I Hind III Nde I Please solve this question in less than 15 minutes for genetics engineering Sal I Plasmid Handout DNA Sequence (both strands are represented) GIGATCC CCTAG G U G|AATTC CTTAA G GTT | AAC CAAjTTG A|AGCTT TTCGA | A CATATG GTATAC G|TCGAC CAGCT | G agtgacata tga ttcgag c cggta a c tca ctgtat a ст aagctcgagccattg cggggat cctctag agtcgacctgcagg c gcccctaggagatctcagctgga cgtc cg tagca ag c tggcgt at atggta cata at cgttcga accgcattagtaccat gta t gggatceptet ct ccagtaggtaggc cgtcg ccct agga aga g g t catccat cc ggcag c Antibioty Resistance gene gc taggetta a actgggatccat gcc origin o tplgm cg at cc a at ttgaccctaggta cgplasmid replication What is the role of the plasmid ? Explain the experimental details of the transformation. How can the success of transformation be observed ?
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