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What is the size in µm of a cell that is 135mm wide in my photo?
The scale bar in the photo is 2.8cm long and represents 8µm.
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- FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification? 2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?The culture you are working has a doubling time of 2 hours and a cell density of 3 x 106 cells per mL. For your experiment, you first dilute the culture 100 fold. Assuming that there is no lab phase and that the cells remain in exponential growth the entire time, what is the cell density (cells/mL) after 10 hours?
- Calculate the width of all 3 cells in micrometers. Use scale. Of a 40x objective.In your opinion, for efficient multiphoton excited fluorescence microscopy, would it be better to use a 100 fs 80 MHz laser pulse train or a 200 fs 80 MHz laser pulse train for imaging biological samples? Please explain your answer using equations 1 and 2 <I(t)2> = gP <I(t)>2 / (Rτ) ..............equa (1) τout = τin (1 + 7.68(D/τ2in)2) 1⁄2 ................equa (2) D is the total dispersion in femtoseconds squared gp is a unitless factor that depends on the temporal laser pulse shape (0.66 for a Gaussian pulse shape), τ is the full-width half-maximum (FWHM) of time average valueWhy do we use sucrose solution when we observe plant cells? What explanation please..
- Explain why you can use OD675 (optical density at a wavelength of 675nm) as a proxy for cell density of your algal cultures.If the volume of a Staphylococcus aureus cell is estimated at 0.5 μm3, how many cells could be accommodated, in principle, in 5 mL of saturated culture? (1 mL = 1 cm3). Show your calculations.Show the calculations required to make up 250mlof a stock solution of this chemical that will then be at the working concentration when 1 volume of this solution is added to 10 volumes of cell culture medium.
- Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that a bacterial culture contains 7.4 x 109 CFUs/ml. What dilution of this sample would give you a countable plate?the optical density of your cell culture is 0.92. How much of this culture should you add to 500 mL of LB to obtain a starting optical density of 0.1?A 2x10-6 dilution of your bacterial culture yielded 10 colonies. What was the cell density (incells/ml) of your bacterial culture?