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You were working with mouse mitochondrial proteins, and as a routine, you have isolated muscle tissues and homogenized it in ice-cold conditions. Following homogenization, purification was carried out by differential velocity centrifugation, equilibrium density gradient centrifugation, and finally, you have collected the fractions. But you have failed to detect any mitochondrial marker enzyme citrate synthase activity in the fractions. When you visualized the samples under the microscopes, you observed broken organelle debris due to severe proteolytic degradation. Which of the following statement may justify the cause of this result?

You were working with mouse mitochondrial proteins, and as a routine, you have isolated muscle tissues and homogenized it in ice-cold conditions. Following homogenization, purification was carried out by differential velocity centrifugation, equilibrium density gradient centrifugation, and finally, you have collected the fractions. But you have failed to detect any mitochondrial marker enzyme citrate synthase activity in the fractions. When you visualized the samples under the microscopes, you observed broken organelle debris due to severe proteolytic degradation. Which of the following statement may justify the cause of this result?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You were working with mouse mitochondrial proteins, and as a routine, you have isolated muscle tissues and homogenized it in ice-cold conditions. Following homogenization, purification was carried out by differential velocity centrifugation, equilibrium density gradient centrifugation, and finally, you have collected the fractions. But you have failed to detect any mitochondrial marker enzyme citrate synthase activity in the fractions. When you visualized the samples under the microscopes, you observed broken organelle debris due to severe proteolytic degradation. Which of the following statement may justify the cause of this result? You have probably forgotten to add protease in the homogenization buffer You have probably forgotten to add phenylmethylsulfonyl fluoride (PMSF) into the homogenization buffer You have probably forgotten to add trypsin and EDTA into the homogenization buffer All of the other answer choices can justify your experimental result
Transcribed Image Text:You were working with mouse mitochondrial proteins, and as a routine, you have isolated muscle tissues and homogenized it in ice-cold conditions. Following homogenization, purification was carried out by differential velocity centrifugation, equilibrium density gradient centrifugation, and finally, you have collected the fractions. But you have failed to detect any mitochondrial marker enzyme citrate synthase activity in the fractions. When you visualized the samples under the microscopes, you observed broken organelle debris due to severe proteolytic degradation. Which of the following statement may justify the cause of this result? You have probably forgotten to add protease in the homogenization buffer You have probably forgotten to add phenylmethylsulfonyl fluoride (PMSF) into the homogenization buffer You have probably forgotten to add trypsin and EDTA into the homogenization buffer All of the other answer choices can justify your experimental result
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