What method of bacterial measurement does this graph represent? Is it considered direct, or indirect? What phase of the growth curve can NOT be measured using this method?

Q: What is the purpose of flaming an inoculating loop?  How will you know when you have flamed the loop…

A: Hi, since you have posted multiple questions, we will be providing you with answers for the first…

Q: Using one of the graphs obtained with the medium II, name the different phases of the bacterial…

A: Micro organisms are typically grown in a closed culture media. Nutrients are added according to the…

Q: In spread plate method, colonies form within the agar and agar surface. In pour plate method,…

A: Introduction Agar media is essential for the study of microorganisms and molecular biology, It is a…

Q: Can you tell IF the pasteurization process is working in this milk producing plant? How can you…

A: Pasteurization is a process in which milk or milk product is heated to a certain temperature for a…

Q: What is the differential agent in EMB medium? a. lactose  b. glucose c. phenol red

A: There are different media available for the growth of microorganisms: Basal media, enriched media,…

Q: For the following methods of bacterial enumeration, does the method determine total count or viable…

A: Bacterial enumeration is counting the number of bacterial cells present in the given sample. Total…

Q: Clostridium tetani is a common soil bacterium and causes tetanus. Would you expect tetani to possess…

A: Clostridium tetani is a gram-positive sporulating bacterium. It is found in soil and forms…

Q: Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the…

A: The term sterilization refers to any process that eliminates, kills, removes, or deactivates all…

Q: Why is glucose not the choice of carbohydrate for most differential media used in the identification…

A: Bacilli present in the intestinal tract are rod shaped bacteria.for example, enteric bacteria…

Q: How would a limited food supply affect the bacterial growth curve? The lag phase would be longer…

A: Bacterial growth curve is the number of living cells plotted against time. It has four phases, these…

Q: What is culture media? Which culture media is used for gram negative bacteria and which one for gram…

A: Microbiology is the branch of science which deals with the study of different types microorganisms…

Q: hat are the components of a carbon-free broth or minimum medium for Bacillus subtillus ? An example…

A: Nutrient media is used for the growth of different microorganisms in laboratory.

Q: Describe the bacterial colonies providing information on shape, color, size, elevation and edge…

A: *morphology of Escherichia coli is identified as rough or a smooth form. *The trough  forms colonies…

Q: If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to…

A: What is motility? Motility is the ability of an organism to move by itself. Eucaryotic cells use…

Q: Why is it important to follow sterile technique?

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: You were given a mixed nutrient agar broth culture of bacteria  1a. How will you determine the…

A: A mixed bacterial culture consists of more than one bacterial cell types growing together. On the…

Q: What is the correct shape of the unknown bacterium growing on your mannitol salt agar

A: Mannitol salt agar also known as MSA is commonly used selective agar in microbiology. This material…

Q: Is  Bacillus cereus negative or positive for growing in high salt concentrations?

A: Bacillus cereus is a gram-positive, endospore forming, non-capsulated bacillus, motile with…

Q: How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial…

A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…

Q: How is the CTSA (coconut water tryptic soy agar) growth medium designed to meet the nutritional need…

A: Many different kinds of media are formulated in the microbiology lab to support the growth of a wide…

Q: What is the most commonly used fixative in stool samples and why? Why is staining of stool samples…

A: What is the most commonly used fixative in stool samples and why? Why is staining of stool samples…

Q: Which of these bacterial characteristics could be evaluated with one of the rapid identification…

A: Arabinose Fermentation Test is used to determine the if the microbes can ferment the carbohydrate…

Q: For the following methods of bacterial enumeration does the method determine total count or viable…

A: MPN (i.e. Most probable number), microscopic count , standard plate count, turbidity is type of…

Q: Are you able to identify the lag, log, stationary and death phase in using this growth curve?

A: Microbes grown in a closed or batch culture in which  certain conditions are that no nutrients are…

Q: Why are the streak plates incubated at 7°C when selecting for psychrotrophic bacteria?

A: Bacteria are unicellular, prokaryotic organisms which are devoid of membrane bound organelles and a…

Q: why are psychrotrophic bacteria incubated at 7 degrees

A: Each species of microbe has a preferred temperature that lies somewhere between its characteristic…

Q: Do Gram-positive bacteria grow on PEA agar? If not, what prevents their growth?

A: Bacteria are common, mostly free-living organisms that are often made up of only one biological…

Q: Which of the following tests can be used to distinguish between Enterobacter and Pseudomonas…

A: Introduction  Small, single-celled organisms are known as bacteria. Nearly every place on Earth…

Q: How does Pseudomonas aerogenosa contribute to the virulance of the pathogen?

A: Pseudomonas aeruginosa is a bacterium which is liable for serious infectious diseases, chronic…

Q: A laboratory technician performed a series of 10 fold serial  dilutions shown below. he plated 0.1…

A: In this question, we are given colony count from different 10 fold dilutions. The volume plated from…

Q: Is the Mueller-Hinton Agar (MHA) a complex or defined medium? Explain based on its composition. Is…

A: Mueller and Hinton in 1941 developed Mueller Hinton Agar(MHA). MHA is used for routine…

Q: To determine the number of cells in a pure culture of Klebsiella pneumoniae, you have performed a…

A: Colony-forming units per millilitre (cfu/ml) of Klebsiella pneumonia original culture can be found…

Q: TRUE OF FALSE: Is E coli colonies colorless in Eosin Methylene Blue Agar (EMBA)? Coliform colonies…

A: Answer: Introduction: Bacteria inhabiting or predominantly present the human intestine is…

Q: Agar is a complex polysaccharide obtained from seaweed and cannot be digested by most…

A: Growth media is the nutrients rich medium that helps in the growth of several bacteria and other…

Q: Why would the reduction in the viable norovirus be much larger in a phosphate buffer solution than…

A: Norovirus is a type of virus that belongs to the family of Caliciviridae. It is commonly called the…

Q: What is the motivation behind the use of stab slant and surface inoculation

A: Agar slants are prepared by adding the agar media to the test tube and allowing it to cool in a…

Q: Why does uninoculated agar plates still has visible growth of microorganisms? Where do you think the…

A: Microorganisms are present in abundance in nature. When there are no aseptic conditions then…

Q: What are some factors used to determine the BSL necessary for working with a specific pathogen?…

A: Answer 1)  BSL is(Biosafety Level)  determined by agent's infectivity , its ease of transmission ,…

Q: What microbe requires serum components to be added to the growth medium?

A: Introduction Microorganisms:- An organism that can be seen only through a microscope, They live in…

Q: A pure culture of an unknown bacterium was streaked onto plates of a variety of media. You notice…

A: A culture medium is a medium that is used to provide support to the growing microorganisms. This…

Q: Identify and explain the different parts of the bacterial growth curve Please explain with as LITTLE…

A: A bacterial growth curve study requires the cultivation of viable cells in a fresh sterile medium,…

Q: Why is aseptic urine collection important when cultures are ordered? If you counted 20 colonies…

A: Urine collection for the urinary tract infection.

Q: 1. Differentiate between defined and undefined culture media. 2. Define alpha, beta, and…

A: The culture medium is a solid, semi-solid or liquid substrate that is rich in nutrients and other…

Q: Why do we grow the bacteria in minimal media when testing for citrate utilization

A: Minimal media are those that provide the minimal nutrients possible for colony development, usually…

Q: James inoculated a species of bacteria into KIA media and realized that it has a yellow butt and a…

A: Introduction KIA or the Kilger Iron Agar is a differential media that helps in distinguishing the…

Q: How is the CTSA (coconut tryptic soy agar plate) growth medium designed to meet the nutritional need…

A: TSA is a general-purpose plating medium that can be used to isolate, cultivate, and maintain a wide…

Q: What is the correct arrangement of the unknown bacterium growing on your mannitol salt agar? Also,…

A: Mannitol Salt Agar (MSA) is a selective as well as differential medium, used for selective isolation…

Q: A microbiologist is working with a pure strain of bacteria isolated from a mixed sample. Which of…

A: Introduction Helicobacter pylori, formerly known as Campylobacter pylori, is a spiral (helical)…

Q: Bacteria can increase in their number by binary fission into two daughter cells. The rate of…

A: The bacterial growth curve is divided into the four-phase the lag phase, the log phase or…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.