What reaction was catalyzed by salivary amylase? Part 1. Effect of pHThe pH of an enzyme's environment can affect its activity by interfering with the charge on itsamino acids. Small changes in pH can result in enzyme denaturation and subsequent loss of catalyticactivity. Each enzyme has a characteristic optimum pH which usually falls within the physiological pHrange. Note that for the purpose of this experiment, distilled water has a pH of -7.00.Watch this video and answer the related questions in the questions section of this activity.The experiment in the video follows this procedure:1. Prepare a water bath maintained at 37°C.2. Mix the components shown in the table below:Test Tube1% Starch0.2M NacIAcid/ Base/ WaterSalivarySolutionAmylaseA10 mL0.5 mL1 mL 0.05 M HCI2 mLВ10 mL0.5 mL1 mL distilled water2 mLC10 mL0.5 mL1 mL 0.05M NaOH2 ml3. Place the test tubes in a water bath.4. Prepare a spot plate that contains two drops of I, in KI.5. When the temperature of the test tube contents is 37°C, remove a few drops from the testtubes and place them in the corresponding spot plates.6. Withdraw another two drops every two minutes until a spot shows no color change.Part 2. Effect of TemperatureAs the temperature of an enzymatically catalyzed reaction increases, so does the speed of thereaction. However, when the temperature goes beyond a certain point, it causes adverse effects onthe enzyme's tertiary structure. As a result, enzyme activity slows down. The temperature that allowspeak enzyme activity is known as the optimum temperature for that enzyme.Watch this video and answer the related questions in the questions section of this activity.The experiment in the video follows this procedure:1. Based on the results from part 1, choose the mixture that showed high amylase activity.2. Mix the components of this mixture in a different test tube except for the salivary amylasewhich should be placed in a separate, smaller test tube.3. Let the two test tubes equilibrate in a water bath maintained at 4°C for 10 minutes.4. After 10 minutes, mix the salivary amylase with the other components.5. Prepare a spot plate with 9 of its wells containing two drops of I, in Kl each.6. Remove a few drops from the test tubes and place them in the corresponding spot plate.7. Withdraw another two drops every two minutes until all wells with I, in Kl are filled.Repeat the same process but at 37°C and in 70°C.

Starch is a polysaccharide, a storage form of glucose in the plant cells. Vegetables, fruits, and…

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Science

Biochemistry

What reaction was catalyzed by salivary amylase?

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter7: Energy And Metabolism

Section: Chapter Questions

Problem 11TYU: PREDICT In the following reaction series, which enzyme(s) is/are most likely to have an allosteric...

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