What would be the effect of raising the column temperature on the retention rate?

Q: Column Chromatography works by the same principle as TLC however the silica gel is not plated on a…

A: Note : Compound A is more soluble in mobile phase. Therefore less polar and having higher Rf.…

Q: Which of the following factors DOES NOT affect retention time of a sample undergoing normal phase…

A:

Q: Suppose the distance a compund moved in a paper chromatography experiment was 30 millameters and the…

A: We have to calculate the Rf value when in paper chromatography, the distance moved by the compound…

Q: Which of the following is TRUE for a reversed phase chromatography experiment? i. Less polar…

A: In Reverse phase chromatography, mobile phase is polar and stationary phase is the non-polar…

Q: Explain how the Rf (Retention Factor)-values can be in agreement or disagreement with the “like…

A: Interpretation -  To explain all about that how the Rf ( Retention factor ) - values can be…

Q: What would happen if we use longer columns and select adsorbing materials with 200-400 mesh sizes…

A: Solution is given in the following steps:

Q: The identity and ratio of solvents are necessary in chromatography for achieving a good separation.…

A: if your solvent had an overly polar TLC developer then all the components of the mixture will move…

Q: Consider a sample that is a mixture composed of biphenyl, benzoic acid, and benzyl alcohol. Predict…

A: Chromatography is a separation technique in which mixture of organic compounds are separated. It…

Q: Capillary electrophoresis differs from other chromatographic methods because:

A: It uses unique detectors.

Q: It is not of the solute property detector used in HPLC is: a. Refractive index detector b. UV…

A: Mixture components are estimated using HPLC detectors. The actual separation in HPLC (High…

Q: a) Discuss the effect of solvent polarity on Rf-values. b)Explain how the Rf (Retention…

A: Since you have posted two questions, we will solve first question for you. Please repost other…

Q: Why do we need to add sand on top of the silica in column chromatography? What could happen if we…

A: The presence of different compounds in the mixture is separated by employing the separation methods.…

Q: In chromatography, if the temperature of oven increases, what will be its effect on retention time…

A: Chromatography It is the separation and purification technique used in which a stationary phase and…

Q: Outline the principle of the supercritical fluid chromatography technique .

A: Supercritical fluid chromatography encompasses all chromatographic methods in which the mobile phase…

Q: Mention 2 advantages of a capillary column as compared to a packed column

A: Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: What would happen to the retention time of a compound if the followingchanges were made?a. Decrease…

A: Chromatography is the technique to separate components of a mixture.

Q: Why is it important to mark the solvent level on the chromatography paper as soon as you remove it…

A: We know that, it is important to mark the solvent level on the chromatography paper as soon as it is…

Q: What controls the mobile phase flow rate in ascending TLC or paper chromatography? Do you think it…

A: The answer is given below

Q: Explain how solid-phase microextraction works. Why is cold trapping necessary during injection with…

A:

Q: In a gas chromatographic run, anthracene peak was eluted at 7.50 min. The adjusted retention time of…

A: Hii there, As there are multiple question posted. we are answering first question. If you need…

Q: do you think that a dye with a high retention factor is more soluble or less souluble in the solvent…

A: Retention factor is defined as the ratio of the distance travelled by the solute solution to the…

Q: How will the following changes affect the retention time of the solute in supercritical fluid…

A: Supercritical fluid chromatography (SFC) is an analytic techniques used for separation similar to…

Q: Which of the following is NOT a factor that affects GC retention time? Select an answer and submit.…

A: As per our guidelines, we are supposed to answer?️ only one question. Kindly repost other questions…

Q: Suggest a reason why the optimum linear flow rate is much higher in gas chromatography than in…

A: Given:-             The optimum linear flow rate is much higher in gas chromatography than in liquid…

Q: The eluent is to be below the 1.5 cm line on the chromatographic paper. Describe the expected…

A: Introduction- Thin layer chromatography(TLC) is a separation technique where the compounds are…

Q: Doe hydrophobic silica gel act as non-polar stationary phase in TLC making the process as reverse…

A: Reversed  phase chromatography  have  long chain alkyl chains which are non polar in nature .

Q: What accounts for a high

A: Chromatography is a technique used for separation and identification of the components in mixture…

Q: A sample that contained two components was separated on a column. The column had a length of 15.0…

A: Given: length of column = 15 cm initial resolution = 1.2 required resolution = 2.0 we have to find…

Q: Why does the efficiency (decreased plate height) of liquid chromatography increase as the stationary…

A: to improve resolution in HPLC  by Increasing column length. by Decreasing particle size. by…

Q: What would be the effect of increasing the carrier gas flow rate on the retention rate?

A: Retention time is the distance that the compound cover from the time of injection till the maxima in…

Q: What are the properties that determine retention time in the Chromatography process?

A: In chromatography, retention time is the interval between the injection of a sample and the…

Q: Which of the following factors DOES NOT affect retention time of a sample undergoing normal phase…

A: Retention time is the time period where a sample spend on column. Here we have to tell which factor…

Q: Type of guard column, separation column, and suppressor used for anion- exchange chromatography?

A: Anion exchange chromatography is a process that  work for the separation of substance based on their…

Q: (iii) What would be the effect on the retention time and order of eluting if the C18 column is…

A: Answer. C18 column has 18 carbon atoms, having a longer carbon chain makes it's retention time…

Q: a pigment in the thin layer chromatography traveled beyond or farther than the solvent front.…

A: As a pigment in the thin layer chromatography traveled beyond or farther the solvent front therefore…

Q: An orange compound was added to the top of a chromatography column. Solvent was added immediately,…

A: An orange compound was added to the top of a chromatography column. The solvent was added…

Q: applications

A: Let us discuss the principle of size exclusion chromatography: Here the selection of molecules is…

Q: Question a) Discuss the effect of solvent polarity on Rf-values.

A: Since you have posted two questions, we will solve first question for you. Please repost other…

Q: If the stationary phase and the compound being tested are both polar, will the retention factor (Rf)…

A: Given that both the stationary phase and the compound being tested are polar.

Q: How might the Rf values of colorless substances be determined? That is, how could the colorless…

A: we use locating agents which reacts with the substance to form colored compound

Q: Do you expect the more polar component of the mixture to elute first or last during column…

A: Chromatography is a widely used technique to separate different components rom the mixture. During…

Q: How does increasing the polarity of the developing solvent affect the Rf of a compound on a silica…

A: TLC stands for Thin Layer Chromatography. This process is basically used for the differentiation of…

Q: With the methods and GC columns used in organic labs, the volatility of the nitrotoluene products is…

A: The question is based on the concept of gas chromatography. we having given three compounds. we have…

Q: 5. Column Chromatography works by the same principle as TLC however the silica gel is not plated on…

A: To answer question 5.

Q: In the second paragraph you should define and describe the process of gas chromatography. In this…

A: A diluted sample in used in gas chromatography because this helps in establishing a proper…

Q: Determine the order of inhibition time of hexane, hexanol and benzene compounds in the reverse phase…

A: Given compounds: Hexane, hexanol and Benzene. To determine: The order of Inhibition time in Reverse…

Q: How GC retention time can be affected by the mobile phase flow rate and the temperature of the…

A:

Q: What is the purpose of solid-phase extraction? Why is it advantageous to use large particles (50 mm)…

A: An extraction method where compounds are suspended (or) dissolved in mixture of liquid are isolated…

Q: Gel permeation chromatography depends on the degree of species segregation of a sample

A: Given         Gel permeation chromatography depends on the degree of species segregation of a sample…