What would happen to the observed and specific rotation values if you were to halve the path length of the cell used while keeping the concentration the same?
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What would happen to the observed and specific rotation values if you were to halve the path length of the cell used while keeping the concentration the same?
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- If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?What will happen if the concentration gradient is high inside the cell and low outside the cell? Explain why the image is applicable in this process.using the cell potential table found in the Reference Material, why do you get holes in your aluminum foil if you use it to cover lasagna in a steel pan? Hint: Steel is made up of mostly iron and lasagna serves as an acidic medium.
- An additional dark control is recommended for membrane analysis, one that includes the membrane disrupting agent. Why might you want this added control reaction?In your opinion, for efficient multiphoton excited fluorescence microscopy, would it be better to use a 100 fs 80 MHz laser pulse train or a 200 fs 80 MHz laser pulse train for imaging biological samples? Please explain your answer using equations 1 and 2 <I(t)2> = gP <I(t)>2 / (Rτ) ..............equa (1) τout = τin (1 + 7.68(D/τ2in)2) 1⁄2 ................equa (2) D is the total dispersion in femtoseconds squared gp is a unitless factor that depends on the temporal laser pulse shape (0.66 for a Gaussian pulse shape), τ is the full-width half-maximum (FWHM) of time average valueIf the potato cells were hypotonic to the surrounding solution, the surrounding solution itself would be what to the potato cells (hypertonic, hypotonic, or isotonic)?
- Anthocyanins are another type of plant pigment that are purple or red. Say you run a chromatography experiment using the same setup as you did in this lab, and you find that anthocyanins do not move from the line of origin. Assuming the solvent traveled the same distance as in your experiment (D in Table 1 above), what is the Rf value of anthocyanin?For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions. What are the dilutions of the last positive tube and first negative tube respectively? Show computation.Water will always move from an area of high water potential to low water potential. If you drop a cell with WP = 0 into the water with WP = -0.9, then what will happen to the cell?
- Suppose you turn on your power supply to run the gel and find that the milliamps reading is close to zero. What would you check?Determine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis.When the potato cube is surrounded by a hypotonic solution, what change in mass occurs in the potato cells?