(11) If you design a gRNA with the sequence, GGU AUC AUU GCA CUG ACC AG, in theory which position of the nucleotide in the genomic DNA sequence below will be cut by Cas9 protein, which may lead to a knockout due to an error in DNA repair? The starting and the end coordinate positions of the DNA fragment on the chromosome are indicated at the beginning and end of the sequence, respectively.

Biology 2e
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ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter14: Dna Structure And Function
Section: Chapter Questions
Problem 37CTQ: An adult with a history of tanning has his genome sequenced. The beginning of a protein-coding...
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(11) If you design a gRNA with the sequence, GGU AUC AUU GCA CUG ACC AG, in theory which
position of the nucleotide in the genomic DNA sequence below will be cut by Cas9 protein, which may
lead to a knockout due to an error in DNA repair? The starting and the end coordinate positions of the
DNA fragment on the chromosome are indicated at the beginning and end of the sequence,
respectively.
110,001 ATC GGT ATC ATT GCA CTG ACC AGA GGC TAC 110,030
A) Between 110,023 (G) and 110,024 (A)
B) Between 110,021 (C) and 110,022 (A)
C) Between 110,020 (C) and 110,021 (C)
D) Between 110,009 (C) and 110,010 (A)
Transcribed Image Text:(11) If you design a gRNA with the sequence, GGU AUC AUU GCA CUG ACC AG, in theory which position of the nucleotide in the genomic DNA sequence below will be cut by Cas9 protein, which may lead to a knockout due to an error in DNA repair? The starting and the end coordinate positions of the DNA fragment on the chromosome are indicated at the beginning and end of the sequence, respectively. 110,001 ATC GGT ATC ATT GCA CTG ACC AGA GGC TAC 110,030 A) Between 110,023 (G) and 110,024 (A) B) Between 110,021 (C) and 110,022 (A) C) Between 110,020 (C) and 110,021 (C) D) Between 110,009 (C) and 110,010 (A)
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