What's the amount of protein if the measurement at A280 = 1.120, dilution factor is 100 and total volume of extract is 45
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What's the amount of protein if the measurement at A280 = 1.120, dilution factor is 100 and total volume of extract is 45
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- Calculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.197, taking into account the dilution factor 100 per and total volume of extract 140mlA stock solution contains 0.2 mg/mL protein. From this stock solution, 179 uL is diluted with 1.1 mL buffer. What is the concentration of protein in this dilution?Calculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.636, taking into account the dilution factor as per question 6 (100ul) and the total volume of extract as per question 4 (140ml). Give your answer as a number with no decimal places.
- One tenth mL of a protein solution was diluted with 2.5 mL of water. The A280 of the diluted solution was 0.25. How many mL of the original protein solution and water should be mixed to make 1.0 mL of solution of A280 = 0.80?Wh are doing this procedures can you explain? (ex. heating or adding chemicals etc.) 1) Purification of Vitelline from Egg Yolk-Experimental ProcedureMeasure the volume of 3 egg yolks and mix by adding an equal volume of NaCl solution.Extract the mixture with 3-fold volumes of ether and separate the aqueous phase.Do the same procedure 3 times.Mix the sample with water and rinse.It is expected for the protein to collapse.Some more water is added in order to check whether the collapse occurs completely.The sample is centrifuged and the precipitate is dried. 2) Purification of Plasma Albumin and Fibrinogen-Experimental ProcedureAdd ammonium sulfate to 10 ml plasma up to 25% saturation.Separate the collapsed fibrinogen by centrifuge.Increase the saturation level to 33% by adding (NH4) 2SO4.Separate the globulin by centrifugation.Separate prodoglobulins by increasing the saturation level to 46%.Increase the saturation level up to 64% to precipitate albumin.Separate the albumin by centrifuge…The diffusivity of amino acids in polyacrylamide gel is approximately 1x10^-9 cm2./s calculate the initial flux of amino acids, give an instantaneous gradient of (20g/cm 3 )/8cm Why is polyacrylamide gel is used in electrophoresis?
- How do you prepare 50 ml of 20 mg/ml proteinase K from 50 mg/ml stock solutionUsing 25 uL of a patient's serum, how much diluent would be needed to create a 1:2 dilution? ______ ulThe Bradford reagent gives a linear response only from 0.1 mg/mL to 1.4 mg/mL of protein concentration. Would we be able to use an "absorbance vs. concentration" line graph for samples outside of the range indicated? Justify your response.
- Calculate protein concentration in unknown samples 1, 2, 3: Absorbance of Unknown 1 = 0.541 Absorbance of Unknown 2 = 0.85 Absorbance of Unknown 3 = 1.02 Standard Curve: Y = 0.0073xBased on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Seliwanoff’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.You receive an order for “heparin 6,000 units SQ q 12 hrs”. The stock solution contains heparin 5,000 units per 0,5 mL. How many milliliters must be administered?