The Bradford reagent gives a linear response only from 0.1 mg/mL to 1.4 mg/mL of protein concentration. Would we be able to use an "absorbance vs. concentration" line graph for samples outside of the range indicated? Justify your response.
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The Bradford reagent gives a linear response only from 0.1 mg/mL to 1.4 mg/mL of protein concentration. Would we be able to use an "absorbance vs. concentration" line graph for samples outside of the range indicated? Justify your response.
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- What would be the best buffering agent to choose if you wanted to buffer an enzyme reaction or tissue culture medium at pH = 7.95? Group of answer choices a. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid); pK = 7.5 b. Histidine; pK1= 1.78; pK2= 5.97; pK3= 8.97 c. Bicarbonate; pK = 6.1 d. Phosphate; pK1 =2.0, pK2 = 6.8; pK3 = 12.5 e. Tris or Tris(hydroxymethyl)aminomethane; pK = 8.07When eluting your analyte of interest using HPLC, gradient elution can be used. How does Gradient elution differ from Isocratic and comment on the advantages of using this type of mobile phase elution?Compare and contrast the method for determining the minimum inhibitory concentration (MIC) with the Kirby-Bauer disc diffusion test.
- Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If we can order 5 milligrams of fibronectin for $871.00, how much would it cost to have enough to use every well of the 24-well culture plate?How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.During cell couting, when the data are plotted in an ordinary graph or on a cross-section paper, why is the log value of the cell concentration used?
- A 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?give two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm.A gel filtration column was calibrated by measuring elution volumes for two proteins of known mass: glutamate dehydrogenase, 290 kDa with elution volume 20.8 mL; serum albumin, 37 kDa with elution volume 42.5 mL Use these values to determine the equation of the straight line graph that relates log molar mass to elution volume. The elution volume of an unknown protein was 32.2 mL. Use the equation just determined above to estimate the molar mass of the protein in kDa units. Don't round your answer until the end to ensure your answer is within the accepted range. Round your answer to the nearest whole number (e.g. If you calculated 27.52, input your answer as 28).
- If the inoculated lactose broth cube exhibits acid production but without gas formation, will you consider this as a negative or positive result? Explain Why is the MPN test qualitative rather than quantitative? Why is the MPN test qualitative rather than quantitative? Why is MPN test qualitative rather than quantitativeWhat is your overall dilution factor if you complete 3 serial dilutions using a 100-fold dilution each time? (Show your work)Consider the sterilization of a sodium gluconate production medium in the holding section of a continuous sterilizer. Assuming constant temperature, the specific death rate constant of the contaminant is 20 s-1 . If the average residence time in the holding section is 10 seconds, calculate the Del factor for the following and explain the results. i. For Pe = 400 ii. For Pe = 400, assuming plug flow iii. For Pe = 100 iv. For Pe = 100, assuming plug flow