When analyzing a disc diffusion assay using antibiotic discs, you see that the bacteria are growing right up to the disc. This means the ______________ are __________ to the ____________. Select one: a. Bacteria / Sensitive / Antibiotic b. Bacteria / Resistant / Antibiotic c. None of the Above d. Antibiotic / Resistant / Bacteria e. Antibiotic / Sensitive / Bacteria
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When analyzing a disc diffusion assay using antibiotic discs, you see that the bacteria are growing right up to the disc. This means the ______________ are __________ to the ____________.
Bacteria / Sensitive / Antibiotic
Bacteria / Resistant / Antibiotic
None of the Above
Antibiotic / Resistant / Bacteria
Antibiotic / Sensitive / Bacteria
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- The following pictures show the results of a Disk Diffusion Assay for different types of bacteria. For each bacteria, what antibiotic would you recommend be used on the patient? Explain your choice.What are the advantages of real-time PCR over microscopy for diagnosing malaria? a. Giemsa stain is not required for real-time PCR b. It allows for species identification and quantification of malaria-causing plasmodium at lower blood concentration with greater sensitivity and specificity. c. Real-time PCR doesn't require the use of a microscope which is deemed too expensive. d. Real-time PCR has a hiher sensitivity for the main malaria-causing plasmodium, P. falciparum, than microscopy techniques. Thank you!!!1. You are working as a CSI analyst. You collected DNA at a crime scene that is from the suspect but do not have enough to run a test to determine who the suspect is. Please discuss what you would do to attain more of the DNA and how to test the DNA to determine who the suspect is.2. Please discuss how the bacterium Agrobacterium tumefaciens normally functions. Please also discuss how this bacterium can be used to help plants attain genes to help fight off insects and herbicides.
- What is the purpose of the following in a Bradford assay? a. PBS b. the Bradford reagent c. changing the pipette tip between standardsYou perform a Kirby-Bauer assay with two antibiotics. Antibiotic X has a zone of inhibition of 9 mm. Antibiotic Y has a zone of inhibition of 11 mm. Which antibiotic is better at killing this particular microorganism? Group of answer choices 1Antibiotic Y 2Antibiotic X and Y, which have identical antimicrobial activities 3Antibiotic X4 4It is impossible to tell from the information givenWhich of the following is true? a. In one technique, the DNA sequence can be determined using one strand of DNA as it passes through a nanopore. b. Next generation sequencing is slow but accurate. c. Both of the above are true.
- What is the purpose of diluting a culture by making "quadrant streaks" (or "streak plating") bacteria, and what can we use them for? Check all that apply A. To observe colony morphology/color B. To isolate viral plaques C. testing growth conditions or treatments (such as media, antibiotics, incubation temperature etc.,) D. To isolate a clonal population of bacteria from the rest of the culture E. For subculturing (transferring to new media so we can grow more bacteria, storing the plate in a fridge for repeated use so the bacteria don't die quickly) F. Accurate quantification (to calculate CFU/mL) G. To know what strain of bacteria you're working with, since you can tell based on how it looks on the plate. H. To lower risk of contamination/identify contaminationDoes TGF-β treatment cause cells to grow more or less in the soft-agar assay? (a) More, (b) LessWhat can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.
- Choose the one answer that fits best. The pGLO plasmid you used contains an araC gene, a GFP gene and a bla gene. If you performed the exact same experiment with a plasmid that contained a GFP gene and a bla gene, but was missing the araC gene, what would you expect to see on your four plates? a. The positive control would not have any bacterial lawn b. The negative control would have as many colonies as the experimental plate c. The LB/Amp/Ara plate would have some colonies but they would not be fluorescent under UV light d. The experimental plate would not have any colonies e. The LB/Amp plate containing E. coli (+)pGLO would be fluorescentA microbiology student was given a mixed culture of two different gram positive bacteria species to grow into a culture medium using correct aseptic techniques. After two days,one gram positive bacterial species grew on the media and the growth appeared red on the surface of the medium. Tthe second gram positive bacterial species grew and the growth appeared yellow on the surface of the medium. What is the possible explanation for the differences in the color of the bacterial growth? A. the culture was contaminated B. the microbiologist put too much inoculum on the culture medium C. the medium was a selective medium D. the medium was differentialRemember that although there are many interesting ideas about genetic engineering of plants and animals, this is specifically about GE bacteria. Please be sure you are answering the following questions 3) What are the benefits (or potential benefits) of the engineered bacterium? 4) What are the risks (or potential risks) of the engineered bacterium?