What happens if you forget to add Sybrsafe to a gel that will be used for DNA gel electrophoresis a. there will only be bands in your sample lanes b.neither the ladder or your sample lanes will have bands c.your ladder lane will be the only one contanining bands d.the gel will run normally and bands will show up for both the ladder lane and the sample lanes

What happens if you forget to add Sybrsafe to a gel that will be used for DNA gel electrophoresis a. there will only be bands in your sample lanes b.neither the ladder or your sample lanes will have bands c.your ladder lane will be the only one contanining bands d.the gel will run normally and bands will show up for both the ladder lane and the sample lanes

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 2RQ: n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules...

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Similar questions

When working with the gel electrophoresis chamber, what must we keep in mind? A. Keeping the gel flat on the table without any buffer solution B. Not changing your pipette in between DNA samples C. Not shaking the chamber after the DNA has been loaded D. Loading two DNA samples per a single well
What will happen to the concentration and the A260/280 ratio of the DNA sample in the following scenarios? a)Add to low % ethanol. b) Add too little salt. C) Forget to boil the sample
What should you do when loading the DNA sample into the gel well? A. Ensuring that the DNA samples are properly loading into the gel well B. Changing your piper te top in between DNA samples C. Load the DNA ladder into the first time D. All of the above
DNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel? a. The 2000-bp fragment b. The 1000-bp fragment c. The 500-bp fragment d. All will migrate equal distances.
1. a) A technologist has a 20µl sample of DNA and adds 5µl of loading dye before adding the total volume into an agarose gel. What was the original concentration of the loading dye? a. 4x b. 5x c. 6x d. 7x e. None of the above 1. b)A technologist adds 6uL of 5x loading dye to a DNA sample before adding the total volume into the agarose gel. How much DNA sample was combined with the loading dye? 12uL 16uL 20uL 24uL 28uL
When you measured your DNA preparation concentration using the NanoDrop spectrophotometer, it indicated that the DNA concentration was 0.550 ng/uL. Convert this concentration into ug/mL.
Which of the following reagents is NOT correct reagents for the Sanger method of DNA sequencing? a. All four dNTPs b. a small amount of specific ddNTP c. DNA Polymerase d. DNA template to be sequenced e. Pair of primers
you performed a sanger sequencing on a particular segment of DNA. Explain at the molecular level what your gel profile would look like due to this mistake. a. you forgot to add ddNTPs to your test tube. b. you added too much ddNTPs to your test tube
Gel electrophoresis can be used to separate DNA on the basis of: A. the probe used. B. size. C. G and C nucleotide content. D. both size and loading buffer content. E. loading buffer content.
For DNA amplification using PCR to occur, which of the following are needed? A. DNA primers B. thermo stable DNA polymerase C. Helicase D. Choices A and B
Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode? A. DNA is positively charged to attracted to the negative end of the unit B. the agarose gel is positively charged C. the agarose gel in negatively charged D. DNA is negatively charged so attracted to the positive end of the unit
Which of the following is true? a. In one technique, the DNA sequence can be determined using one strand of DNA as it passes through a nanopore. b. Next generation sequencing is slow but accurate. c. Both of the above are true.