When performing a gram stain, it is necessary to remove crystal violet stain from any gram negative bacteria on the slide. Microbiologists use a decolorizing solution to accomplish this. A common recipe is equal parts 95% ethanol and acetone. How many mL of water would you need to add if you have 100% ethanol and want to end up with 300 mL of decolorizer? Report your answer to 2 decimal places and perform all calculations in a singlet step using Excel to avoid accruing rounding error.

Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter1: The Nature Of Chemistry
Section: Chapter Questions
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When performing a gram stain, it is necessary to remove crystal violet stain from any gram negative bacteria on the slide. Microbiologists use a decolorizing solution to accomplish this. A common recipe is equal parts
95% ethanol and acetone.
How many mL of water would you need to add if you have 100% ethanol and want to end up with 300 mL of decolorizer?
Report your answer to 2 decimal places and perform all calculations in a singlet step using Excel to avoid accruing rounding error.
Transcribed Image Text:When performing a gram stain, it is necessary to remove crystal violet stain from any gram negative bacteria on the slide. Microbiologists use a decolorizing solution to accomplish this. A common recipe is equal parts 95% ethanol and acetone. How many mL of water would you need to add if you have 100% ethanol and want to end up with 300 mL of decolorizer? Report your answer to 2 decimal places and perform all calculations in a singlet step using Excel to avoid accruing rounding error.
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